Low-power high-resolution solid-state NMR of peptides and proteins
Low-power high-resolution solid-state NMR of peptides and proteins
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DOI：
10.1021/ja0494510
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发表时间：
2004-04-21
影响因子：
15
通讯作者：
Meier, BH
中科院分区：
文献类型：
--
作者：
Ernst, M;Meier, MA;Meier, BH
我们证明了二维固体核磁共振化学位移相关谱可以在低功率条件下记录。除了交叉极化周期，没有使用高于40 kHz的射频场幅度。这样的实验需要使用快速（>50 kHz）魔角旋转（MAS）。与高功率版本的实验相比，没有显示一般的线加宽，但在偏振传输动力学的一些变化。
We demonstrate that two-dimensional solid-state NMR chemical-shift correlation spectra can be recorded under low-power conditions. Except for the cross-polarization period, no rf-field amplitudes above 40 kHz are used. Such experiments require the use of fast (>50 kHz) magic-angle spinning (MAS). A comparison with the high-power version of the experiment shows no general line broadening but some changes in the polarization-transfer dynamics.