The intragenic cis-elements mediate temperature response of RrKSN
The intragenic cis-elements mediate temperature response of RrKSN
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DOI：
10.1016/j.plaphy.2023.107983
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发表时间：
2023-08-21
影响因子：
6.5
通讯作者：
Wang，Changquan
中科院分区：
文献类型：
--
作者：
Bai，Mengjuan;Wang，Weinan;Wang，Changquan
在许多真核生物中，通过基因内序列进行基因调控已得到越来越多的认识。然而，基因内序列介导的基因表达对环境刺激的响应在很大程度上还没有确定。在这里，我们证明了来自玫瑰‘紫枝’的RrKSN的第一个内含子对RrKSN的表达有正向影响，并且这种影响取决于它的位置和方向。进一步的分析表明，第一内含子中的四个相邻顺式元件(T)CGATT/AATCG(A)对正调控起关键作用，这些元件的突变显著抑制了RrKSN的启动。这些元件进一步被证明是RrARR1的结合位点，RrARR1是拟南芥B型拟南芥反应调节因子1(ARR1)的同源转录因子。在月季幼苗中，第一内含子介导的RrKSN的表达随着RrARR1的过度表达而增强，而在RrARR1沉默时被取消。此外，随着RrARR1的沉默，RrKSN在16℃和28℃之间的表达差异也被消除。综上所述，这些结果表明，RrARR1及其结合元件都是第一次内含子介导的RrKSN表达所必需的，以响应不同的温度。因此，我们的结果揭示了一种独特的基因表达调控机制，植物通过这种机制感知环境温度的信号。
Gene regulation via intragenic sequences is becoming more recognized in many eukaryotes. However, the intragenic sequences mediated gene expressions in response to environmental stimuli have been largely uncharacterized. Here, we showed that the first intron ofRrKSNfrom theRosa rugosacultivar ‘Purple branch’ had a positive effect onRrKSNexpression, and the effect depends on its position and orientation. Further analyses revealed that the four adjacentcis-elements (T)CGATT/AATCG(A) within the first intron were critical for the positive regulation, and theRrKSNpromotion was significantly suppressed with mutations of these elements. Thesecis-elements were further evidenced as binding sites for RrARR1, the homologous of Arabidopsis type-B ARABIDOPSIS RESPONSE REGULATOR 1 (ARR1) transcription factor. The first intron-mediatedRrKSNexpression was enhanced with over-expressing ofRrARR1, but abolished withRrARR1silencing in rose seedlings. Moreover, the expression difference ofRrKSNbetween 16°C and 28°C was eliminated along withRrARR1-silencing. Taken together, these results suggested bothRrARR1and its binding elements are required for the first intron-mediatedRrKSNexpression in response to varying temperatures. Therefore, our results reveal a unique intragenic regulation mechanism of gene expression by which plants perceive the signal of ambient temperature in rose.