Predicting Aggregation Kinetics of DU 145 Prostate Cancer Cells in Liquid-Overlay Culture
Predicting Aggregation Kinetics of DU 145 Prostate Cancer Cells in Liquid-Overlay Culture
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DOI：
10.1007/s10529-005-2725-6
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发表时间：
2005-11
影响因子：
2.7
通讯作者：
K. O'Connor;M. Venczel
中科院分区：
文献类型：
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作者：
K. O'Connor;M. Venczel
一种新的人口平衡模型的预测能力，以模拟附着依赖细胞的聚集动力学在一个细胞增量的分辨率进行了评估。以DU 145人前列腺癌细胞的球状体组装体为代表，用单一的速率常数矩阵，在5× 103 ~ 2.5× 104 cells/cm ~ 2的5倍表面密度范围内，该数学模型被证明是稳健的。对于1× 105个细胞/cm 2的培养物，超过75%的模拟聚集体浓度在测量浓度的标准差内。对于两个极端密度，至少三分之二的模型预测值在实验数据平均值的35%以内。模型预测的误差归因于测量的不确定性和聚集的内在变化。该模型可用于组织工程中球状体的合理设计和制药中的生物分离过程。
The predictive capacity of a novel population-balance model to simulate aggregation kinetics of attachment-dependent cells at the resolution of one-cell increments has been evaluated. Using spheroid assembly of DU 145 human prostate cancer cells as a representative system, the mathematical model proved to be robust in simulating aggregation over a 5-fold range of surface densities from 5×103to 2.5×104cells/cm2with a single matrix of rate constants. For cultures at 1×105cells/cm2, more than 75% of simulated aggregate concentrations are within the standard deviation of measured concentrations. For the two extreme densities, at least two-thirds of model predictions are within 35% of the mean for experimental data. Error in model predictions is attributed to uncertainty in measurements and intrinsic changes in aggregation. The model has application to the rational design of spheroids in tissue engineering and bioseparation processes in pharmaceutical manufacturing.