Molecular imprinting for the recognition of N-terminal histidine peptides in aqueous solution
Molecular imprinting for the recognition of N-terminal histidine peptides in aqueous solution
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DOI：
10.1021/ma020091f
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发表时间：
2002-07-30
期刊：
影响因子：
5.5
通讯作者：
Shea, KJ
中科院分区：
文献类型：
--
作者：
Hart, BR;Shea, KJ
利用分子印迹技术制备多肽大分子受体的新方法已经被开发出来。聚合物受体表现出选择性摄取特定的N-末端组氨酸含有简单的二肽序列。聚合和结合在水中进行。该方法利用强Ni（II）-His结合将二肽的N-末端组氨酸吸引到聚合物表面，并利用肽和聚合物之间的次级相互作用来区分肽序列。这些发展是通过利用水基单体制剂实现的，所述水基单体制剂包括作为水溶性交联单体的N，N '-亚乙基双（丙烯酰胺）和可聚合的NTA配体，所述可聚合的NTA配体可用于将镍和其它金属结合到这些聚丙烯酰胺中。Ni-NTA复合物提供强组氨酸结合位点，其将二肽吸引到聚合物表面。使用低浓度水溶性引发剂和低温的温和聚合条件导致定量聚合物产率。单体组成的变化揭示了最佳的交联，以实现这些聚合物的最大选择性。
A new procedure for creating macromolecular receptors for peptides using molecular imprinting has been developed. The polymeric receptor exhibits selective uptake of specific N-terminal histidine containing sequences of simple dipeptides. The polymerization and binding are carried out in water. The approach utilizes a strong Ni(II)-His binding to attract the N-terminus histidine of the dipeptide to the polymer surface and secondary interactions between peptide and polymer to discriminate between the peptide sequence. These developments are enabled by utilizing an aqueous based monomer formulation that includes N,N'-ethylenebis(acrylamide) as a water-soluble cross-linking monomer and a polymerizable NTA ligand, which can be used to incorporate nickel and other metals into these polyacrylamides. The Ni-NTA complex provides a strong histidine binding site that draws the dipeptide to the polymer surface. Mild polymerization conditions that utilize low concentrations of water-soluble initiator and low-temperature result in quantitative polymer yields. Variation of monomer composition reveals an optimum cross-linking for achieving maximum selectivity for these polymers.