Lactobacillus gasseri LA39 Activates the Oxidative Phosphorylation Pathway in Porcine Intestinal Epithelial Cells
Lactobacillus gasseri LA39 Activates the Oxidative Phosphorylation Pathway in Porcine Intestinal Epithelial Cells
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加氏乳杆菌 LA39 激活猪肠上皮细胞的氧化磷酸化途径
DOI：
10.3389/fmicb.2018.03025
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发表时间：
2018-12-11
影响因子：
5.2
通讯作者：
Yan, Xianghua
中科院分区：
文献类型：
--
作者：
Hu, Jun;Ma, Libao;Yan, Xianghua
肠道微生物与宿主上皮的相互作用对宿主健康具有重要作用。我们之前的数据表明，乳酸杆菌LA39是断奶仔猪肠道中主要的乳酸菌。然而，乳杆菌LA39在仔猪肠上皮蛋白表达中的调节作用尚不清楚。本研究采用比较蛋白质组学方法研究了加氏乳杆菌LA39对仔猪肠上皮蛋白谱的影响。L. gasseri LA39处理后，IPEC-J2细胞中15种蛋白的表达量显著升高，13种蛋白的表达量显著降低。生物信息学分析，包括对差异表达蛋白的COG功能注释、GO注释和KEGG途径分析显示，L. gasseri LA39处理显著激活了IPEC-J2细胞的氧化磷酸化（OXPHOS）途径。进一步的数据表明，与OXPHOS通路相关的两种差异表达蛋白UQCRC2和TCIRG1以及IPEC-J2细胞中ATP水平在L. gas LA39处理后显著上调。重要的是，体内数据表明，灌胃乳杆菌LA39可显著提高断奶仔猪肠上皮细胞中UQCRC2和TCIRG1的表达以及细胞ATP水平。体外和体内实验结果表明，L. gasseri LA39激活OXPHOS通路，增加猪肠上皮细胞的能量产生。这些发现表明，乳杆菌LA39可能是一种潜在的益生菌候选菌，具有促进哺乳动物肠道能量产生和促进健康的功能。
Intestinal microbial interactions with the host epithelium have important roles in host health. Our previous data have suggested that Lactobacillus gasseri LA39 is the predominant intestinal Lactobacillus in weaned piglets. However, the regulatory role of L. gasseri LA39 in the intestinal epithelial protein expression in piglets remains unclear. In the present study, we conducted comparative proteomics approach to investigate the intestinal epithelial protein profile alteration caused by L. gasseri LA39 in piglets. The expressions of 15 proteins significantly increased, whereas the expressions of 13 proteins significantly decreased in the IPEC-J2 cells upon L. gasseri LA39 treatment. Bioinformatics analyses, including COG function annotation, GO annotation, and KEGG pathway analysis for the differentially expressed proteins revealed that the oxidative phosphorylation (OXPHOS) pathway in IPEC-J2 cells was significantly activated by L. gasseri LA39 treatment. Further data indicated that two differentially expressed proteins UQCRC2 and TCIRG1, associated with the OXPHOS pathway, and cellular ATP levels in IPEC-J2 cells were significantly up-regulated by L. gassed LA39 treatment. Importantly, the in vivo data indicated that oral gavage of L. gasseri LA39 significantly increased the expression of UQCRC2 and TCIRG1 and the cellular ATP levels in the intestinal epithelial cells of weaned piglets. Our results, both in vitro and in vivo, reveal that L. gasseri LA39 activates the OXPHOS pathway and increases the energy production in porcine intestinal epithelial cells. These findings suggest that L. gasseri LA39 may be a potential probiotics candidate for intestinal energy production promotion and confers health-promoting functions in mammals.