A simple in vitro Tn7-based transposition system with low target site selectivity for genome and gene analysis
A simple in vitro Tn7-based transposition system with low target site selectivity for genome and gene analysis
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DOI：
10.1093/nar/28.5.1067
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发表时间：
2000-03-01
影响因子：
14.9
通讯作者：
Craig, NL
中科院分区：
文献类型：
--
作者：
Biery, MC;Stewart, FJ;Craig, NL
描述了一种基于Tn7的体外转座系统，该系统表现出很小的靶位选择性，允许有效地回收许多不同的转座子插入到靶DNA中，范围从小质粒到粘粒到整个基因组。描述了两种对基因分析有用的微型Tn7衍生物：一种是用于进行翻译和转录目标基因融合的衍生物，另一种是可以在目标DNA(蛋白质)中插入15个碱基(5个氨基酸)的衍生物。
A robust Tn7-based in vitro transposition system is described that displays little target site selectivity, allowing the efficient recovery of many different transposon insertions in target DNAs ranging from small plasmids to cosmids to whole genomes. Two miniTn7 derivatives are described that are useful for the analysis of genes: one a derivative for making translational and transcriptional target gene fusions and the other a derivative that can generate 15 bp (5 amino acid) insertions in target DNAs (proteins).