The Association of MEG3 lncRNA with Nuclear Speckles in Living Cells.
The Association of MEG3 lncRNA with Nuclear Speckles in Living Cells.
复制标题
MEG3 lncRNA与活细胞核斑点的关联
DOI：
10.3390/cells11121942
复制
发表时间：
2022-06-16
期刊：
影响因子：
6
通讯作者：
Shav-Tal, Yaron
中科院分区：
文献类型：
--
作者：
Hasenson, Sarah E.;Alkalay, Ella;Atrash, Mohammad K.;Boocholez, Alon;Gershbaum, Julianna;Hochberg-Laufer, Hodaya;Shav-Tal, Yaron
核斑点是含有RNA结合蛋白以及RNA（包括长链非编码RNA（lncRNA））的核体。母体表达基因3（MEG3）是一种与核斑点相关的核内滞留lncRNA。为了了解MEG3 lncRNA与活细胞中核斑点的关联动力学，我们产生了可以在真实的时间内检测到的荧光标记的MEG3转录物。在常规条件下，观察到MEG3与核斑点的瞬时关联，包括核质部分。已知影响核斑点结构的转录或剪接失活条件显示MEG3 lncRNA与核斑点的显著和增加的缔合，特别是在核斑点周围形成环状结构。这与转移相关肺腺癌（MALAT1）lncRNA形成对比，后者通常与核斑点高度相关，并在核质中释放和分散。在正常情况下，MEG3与核斑点的周边动态结合，但在转录或剪接抑制下，MEG3也可以进入核斑点的中心。总而言之，使用活细胞成像方法，我们发现MEG3 lncRNA是核斑点的瞬时居民，并且其与该核体的关联受细胞中转录和剪接活动水平的调节。
Nuclear speckles are nuclear bodies containing RNA-binding proteins as well as RNAs including long non-coding RNAs (lncRNAs). Maternally expressed gene 3 (MEG3) is a nuclear retained lncRNA found to associate with nuclear speckles. To understand the association dynamics of MEG3 lncRNA with nuclear speckles in living cells, we generated a fluorescently tagged MEG3 transcript that could be detected in real time. Under regular conditions, transient association of MEG3 with nuclear speckles was observed, including a nucleoplasmic fraction. Transcription or splicing inactivation conditions, known to affect nuclear speckle structure, showed prominent and increased association of MEG3 lncRNA with the nuclear speckles, specifically forming a ring-like structure around the nuclear speckles. This contrasted with metastasis-associated lung adenocarcinoma (MALAT1) lncRNA that is normally highly associated with nuclear speckles, which was released and dispersed in the nucleoplasm. Under normal conditions, MEG3 dynamically associated with the periphery of the nuclear speckles, but under transcription or splicing inhibition, MEG3 could also enter the center of the nuclear speckle. Altogether, using live-cell imaging approaches, we find that MEG3 lncRNA is a transient resident of nuclear speckles and that its association with this nuclear body is modulated by the levels of transcription and splicing activities in the cell.
登录
查看更多内容
影响因子：
14.9
作者：
Greenberg, Eliraz;Hochberg-Laufer, Hodaya;Shav-Tal, Yaron
通讯作者：
Shav-Tal, Yaron
poly（a）RNA的离散核域及其与核的功能组织的关系。
影响因子：
7.8
作者：
Carter, K C;Taneja, K L;Lawrence, J B
通讯作者：
Lawrence, J B
影响因子：
4.4
作者：
Hutchinson, John N;Ensminger, Alexander W;Clemson, Christine M;Lynch, Christopher R;Lawrence, Jeanne B;Chess, Andrew
通讯作者：
Chess, Andrew
DOI：
10.1083/jcb.201807108
发表时间：
2018-11-05
期刊：
The Journal of cell biology
影响因子：
--
作者：
Chen Y;Zhang Y;Wang Y;Zhang L;Brinkman EK;Adam SA;Goldman R;van Steensel B;Ma J;Belmont AS
通讯作者：
Belmont AS
影响因子：
4
作者：
Fei, Jingyi;Jadaliha, Mahdieh;Ha, Taekjip
通讯作者：
Ha, Taekjip