Tributyltin sensitivity of vacuolar-type Na(+)-transporting ATPase from Enterococcus hirae.
Tributyltin sensitivity of vacuolar-type Na(+)-transporting ATPase from Enterococcus hirae.
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海拉肠球菌液泡型 Na() 转运 ATP 酶的三丁基锡敏感性。
DOI：
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发表时间：
2009
影响因子：
2
通讯作者：
Y. Kakinuma
中科院分区：
文献类型：
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作者：
Soracom Chardwiriyapreecha;Tomohiro Inoue;Naoko Sugimoto;T. Sekito;I. Yamato;T. Murata;M. Homma;Y. Kakinuma
三丁基氯化锡(TBT)是一种环境污染物，对多种真核和原核生物具有毒性。一些F-ATP合成酶(F-ATPase)/液泡型ATPase(V-ATPase)超家族成员已被确定为该化合物的分子靶标。TBT对各种生物来源的H(+)转运或Na(+)转运的F-ATPase和H(+)转运的V-ATPase的活性均有抑制作用。然而，转运Na(+)的V-ATPase对TBT的敏感性尚未被研究。我们研究了TBT对一株Hirae肠球菌Na(+)转运V-ATPase的影响。小于10微米的TBT对纯化形式和膜结合形式的平菇V-ATPase的ATP水解性几乎没有抑制作用，其抑制TBT的IC50约为35微米。20微米TBT不能抑制河蟹细胞的主动钠转运，表明该V-ATPase在体内具有活性。而酿酒酵母液泡膜囊泡的H(+)转运V-ATPase的IC50约为0.2微米。因此，E.hirae V-ATPase对TBT的敏感性极低。
Tributyltin chloride (TBT), an environmental pollutant, is toxic to a variety of eukaryotic and prokaryotic organisms. Some members of F-ATP synthase (F-ATPase)/vacuolar type ATPase (V-ATPase) superfamily have been identified as the molecular target of this compound. TBT inhibited the activities of H(+)-transporting or Na(+)-transporting F-ATPase as well as H(+)-transporting V-ATPase originated from various organisms. However, the sensitivity to TBT of Na(+)-transporting V-ATPase has not been investigated. We examined the effect of TBT on Na(+)-transporting V-ATPase from an eubacterium Enterococus hirae. The ATP hydrolytic activity of E. hirae V-ATPase in purified form as well as in membrane-bound form was little inhibited by less than 10 microM TBT; IC50 for TBT inhibition of purified enzyme was estimated to be about 35 microM. Active sodium transport by E. hirae cells, indicating the in vivo activity of this V-ATPase, was not inhibited by 20 microM TBT. By contrast, IC50 of H(+)-transporting V-ATPase of the vacuolar membrane vesicles from Saccharomyces cerevisiae was about 0.2 microM. E. hirae V-ATPase is thus extremely less sensitive to TBT.
Triorganotins 抑制线粒体内膜阴离子通道。
DOI：
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发表时间：
1991
期刊：
The Journal of biological chemistry
影响因子：
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作者：
Powers,MF;Beavis,AD
通讯作者：
Beavis,AD