Role of protein kinase C in the vesicular release of acetylcholine and norepinephrine from enteric neurons of the guinea pig small intestine.
Role of protein kinase C in the vesicular release of acetylcholine and norepinephrine from enteric neurons of the guinea pig small intestine.
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蛋白激酶 C 在豚鼠小肠肠神经元乙酰胆碱和去甲肾上腺素囊泡释放中的作用。
DOI：
10.1254/jjp.48.377
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
C. Tanaka
中科院分区：
文献类型：
--
作者：
S. Hashimoto;H. Shuntoh;K. Taniyama;C. Tanaka
在豚鼠小肠条上研究了蛋白激酶C参与[~ 3 H]乙酰胆碱（ACh）和[~ 3 H]去甲肾上腺素（NE）释放的作用。12-O-十四酰佛波醇13-乙酸酯（TPA）可增强A23187诱发的[3 H]乙酰胆碱和[3 H]去甲肾上腺素（NE）的释放，而4 α-佛波醇12，13-二癸酸酯（4 α-PDD）则无此作用，TPA的增强作用可被多粘菌素B抑制。TPA也能增强高K+诱发的[~ 3 H]ACh和[~ 3 H]NE在河豚毒素存在下的释放。这些TPA诱导的诱发释放的增强作用在低浓度的外部Ca 2+（0.5 mM）比在高浓度（2 mM）更大。哇巴因诱导释放这些神经递质的情况下和存在的低浓度的外部Ca 2+。TPA不改变哇巴因诱发的释放。上述结果表明，蛋白激酶C的激活增强了豚鼠小肠肠神经元神经末梢在低Ca ~（2+）浓度下释放乙酰胆碱和去甲肾上腺素的能力。
The involvement of protein kinase C in the release of [3H]acetylcholine (ACh) and [3H]norepinephrine (NE) was studied in strips of guinea pig small intestine. 12-O-tetradecanoyl phorbol 13-acetate (TPA), but not 4 alpha-phorbol-12,13-didecanoate (4 alpha-PDD) potentiated the A23187-evoked release of [3H]ACh and [3H]NE from the strips of small intestine preloaded with [3H]choline and [3H]NE, and the potentiating effect of TPA was inhibited by polymyxin B. High K+-evoked releases of [3H]ACh and [3H]NE in the presence of tetrodotoxin were also potentiated by TPA. These TPA-induced potentiations of the evoked release were greater at a low concentration of external Ca2+ (0.5 mM) than at a high concentration (2 mM). Ouabain induced the release of these neurotransmitters both in the absence and presence of the low concentration of external Ca2+. The ouabain-evoked release was not altered by TPA. These results indicate that the activation of protein kinase C potentiates the vesicular release of ACh and NE at low Ca2+ concentration from the nerve terminals of enteric neurons in the guinea pig small intestine.
DOI：
10.1073/pnas.79.17.5249
发表时间：
1982-09
影响因子：
11.1
作者：
W. Wu;S. Walaas;A. Nairn;P. Greengard
通讯作者：
W. Wu;S. Walaas;A. Nairn;P. Greengard