Problems in the assessment of the package effect in five small phytoplankters
Problems in the assessment of the package effect in five small phytoplankters
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五种小型浮游植物组合效应评估中存在的问题
DOI：
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发表时间：
1989
期刊：
影响因子：
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通讯作者：
R. Geider
中科院分区：
文献类型：
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作者：
B. Osborne;R. Geider
通过比较完整细胞和破坏细胞的吸收光谱，评估了5种小型浮游植物（Thalassiosira sp.，克隆2601（一种未识别的真核生物），Nannochloris atomus， syns和Synechococcus WH 7803）的包裹效应的大小。包效应随着细胞尺寸的减小而显著减小，这与基于Mie理论的理论预测基本一致。然而，由于无法将λ=750 nm处的衰减（表观吸收）测量值分配给散射或吸收，对封装效应的定量评估受到了干扰。在λ=750 nm处，最小浮游生物的表观吸收幅度最大，并且在细胞破坏后减少，但没有消除。虽然λ=750 nm处的表观吸收通常被认为是由于残余散射损失造成的，但现有证据并不排除这可能部分是由于细胞或细胞成分的吸收造成的，这需要进一步研究。尽管这些困难在小型浮游生物中尤为明显，但没有理由认为它们不会使对大型浮游植物细胞的包装效应的评估复杂化。
The magnitude of the package effect in five small phytoplankters [Thalassiosira sp., Clone 2601 (an unidentified eucaryote), Nannochloris atomus, Synechococcus ‘Syn’ and Synechococcus WH 7803] was assessed by comparison of the absorption spectra of intact and disrupted cells. The package effect was considerably reduced with reductions in cell size and this was broadly in agreement with theoretical predictions based on Mie theory. However, the quantitative assessment of the package effect is confounded by an inability to assign attenuation (apparent absorption) measurements at λ=750 nm to either scattering or absorption. The magnitude of the apparent absorption at λ=750 nm was greatest with the smallest picoplankton species examined, and was reduced, but not eliminated, after cell disruption. Whilst the apparent absorption at λ=750 nm is commonly thought to be due to residual scattering losses, the available evidence does not exclude the possibility that this may be due in part to absorption by cells or cell constituents and this requires further examination. Although these difficulties are particularly evident with the small picoplankton species, there is no reason to expect that they will not complicate the assessment of the package effect in larger phytoplankton cells.