In vitro differentiation of human processed lipoaspirate cells into early neural progenitors
In vitro differentiation of human processed lipoaspirate cells into early neural progenitors
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DOI：
10.1097/01.prs.0000055043.62589.05
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发表时间：
2003-05-01
影响因子：
3.6
通讯作者：
Hedrick, MH
中科院分区：
文献类型：
--
作者：
Ashjian, PH;Elbarbary, AS;Hedrick, MH
人加工抽脂细胞（PLA）是一种多能干细胞，能够分化为多种间充质细胞系（骨、软骨、脂肪和肌肉）。迄今为止，分化为非中胚层尚未见报道。本研究表明，聚乳酸细胞可以诱导分化为早期神经祖细胞，这是一个外胚层起源。未分化培养的人聚乳酸细胞表达神经细胞特征标记物，如神经元特异性烯醇化酶（NSE）、波形蛋白和神经元特异性核蛋白（NeuN）。用异丁基甲基黄嘌呤、吲哚美辛和胰岛素处理PLA细胞2周后，约20% ~ 25%的细胞分化为具有典型神经形态特征的细胞，并伴随NSE、vimentin和神经生长因子受体trk-A的表达增加。然而，诱导的聚乳酸细胞不表达成熟神经元标记物MAP或成熟星形胶质细胞标记物GFAP。研究还发现，神经诱导的PLA细胞表现出延迟整流型K+电流（一种早期发育的离子通道），并伴有形态学变化和神经特异性标记物表达增加。作者得出结论，人聚乳酸细胞可能具有在体外分化为代表神经元和/或胶质细胞早期祖细胞的细胞的潜力。
Human processed lipoaspirate (PLA) cells are multipotent stem cells, capable of differentiating into multiple mesenchymal lineages (bone, cartilage, fat, and muscle). To date, differentiation to nonmesodermal fates has not been reported. This study demonstrates that PLA cells can be induced to differentiate into early neural progenitors, which are of an ectodermal origin. Undifferentiated cultures of human PLA cells expressed markers characteristic of neural cells such as neuron-specific enolase (NSE), vimentin, and neuron-specific nuclear protein (NeuN). After 2 weeks of treatment of PLA cells with isobutylmethylxanthine, indomethacin, and insulin, about 20 to 25 percent of the cells differentiated into cells with typical neural morphologic characteristics, accompanied by increased expression of NSE, vimentin, and the nerve-growth factor receptor trk-A. However, induced PLA cells did not express the mature neuronal marker, MAP, or the mature astrocyte marker, GFAP. It was also found that neurally induced PLA cells displayed a delayed-rectifier type K+ current (an early developmental ion channel) concomitantly with morphologic changes and increased expression of neural-specific markers. The authors concluded that human PLA cells might have the potential to differentiate in vitro into cells that represent early progenitors of neurons and/or glia.