Development of a third-generation glucose sensor based on the open circuit potential for continuous glucose monitoring
Development of a third-generation glucose sensor based on the open circuit potential for continuous glucose monitoring
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DOI：
10.1016/j.bios.2018.09.099
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发表时间：
2019-01-15
影响因子：
12.6
通讯作者：
Sode, Koji
中科院分区：
文献类型：
--
作者：
Lee, Inyoung;Loew, Noya;Sode, Koji
连续葡萄糖监测（CGM）系统在当前I型糖尿病护理中是最重要的，并且作为人工胰腺系统开发的组成部分，因为所供应的胰岛素量是基于CGM结果计算的。因此，为了稳定和准确地控制血糖水平，CGM应长期稳定和准确。我们一直致力于FAD依赖的葡萄糖脱氢酶复合物（FADGDH）的生物分子工程和应用，该复合物具有直接电子转移的能力。在这项研究中，我们报告的第三代型开路电位（OCP）的原则为基础的葡萄糖传感器的发展与直接电子转移FADGDH固定在金电极上使用自组装单分子膜（SAM）。我们为基于OCP的葡萄糖传感器开发了一种新的算法。通过采用这种新的算法，实现了测量和传感器制备的高重现性。此外，信号不受样品溶液中存在对乙酰氨基酚和抗坏血酸的影响。优化后的第三代OCP葡萄糖传感器可以连续工作9天以上，而信号、灵敏度和动态范围没有明显变化，表明其在CGM系统中的潜在应用。
Continuous glucose monitoring (CGM) systems are most important in the current Type I diabetes care and as component for the development of artificial pancreas systems because the amount of insulin being supplied is calculated based on the CGM results. Therefore, to stably and accurately control the blood glucose level, CGM should be stable and accurate for a long period. We have been engaged in the biomolecular engineering and application of FAD dependent glucose dehydrogenase complex (FADGDH) which is capable of direct electron transfer. In this study, we report the development of the third-generation type open circuit potential (OCP) principle-based glucose sensor with direct electron transfer FADGDH immobilized on gold electrodes using a self-assembled monolayer (SAM). We developed a novel algorithm for OCP-based glucose sensors. By employing this new algorithm, high reproducibility of measurement and sensor preparation were achieved. In addition, the signal was not affected by the presence of acetaminophen and ascorbic acid in the sample solution. The thus optimized third-generation OCP-based glucose sensor could be operated continuously for more than 9 days without significant change in the signal, sensitivity and dynamic range, indicating its potential application for CGM systems.