A forward genetic screen for genes regulating mineralized tooth and bone formation in zebrafish (Danio rerio)
A forward genetic screen for genes regulating mineralized tooth and bone formation in zebrafish (Danio rerio)
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DOI：
10.1111/j.1439-0426.2010.01403.x
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发表时间：
2010-04-01
影响因子：
0.9
通讯作者：
Connolly, M. H.
中科院分区：
文献类型：
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作者：
Yelick, P. C.;Connolly, M. H.
我们的实验室研究颅面骨骼和牙齿的再生.我们正在使用的一种方法是利用斑马鱼模型，通过大规模的，正向遗传，化学N-乙基亚硝基脲（ENU）诱变筛选，以确定调控矿化颅面，轴和牙齿发育的基因。斑马鱼不断再生牙齿的事实使它们成为研究牙齿再生的非常有用的模型。我们的目标是确定和表征调节这些过程的分子遗传信号通路，这些通路可以通过靶向基因递送策略进行操纵。通过这些努力，我们希望最终确定有效的，临床相关的人类骨骼和牙齿替代疗法的方法。在这里，我们描述了我们的研究使用斑马鱼模型，这被证明是有用的识别和表征的基因调控矿化组织的形成，再生和稳态。虽然目前是初步的，但我们预计将阐明调节骨和牙齿再生的新信号通路，这将最终促进人类骨骼和牙齿发育不良的修复。
P>Our laboratory studies craniofacial skeletal and tooth regeneration. One approach we are using is to exploit the zebrafish model via a large-scale, forward genetic, chemical N-ethyl-nitroso-urea (ENU) mutagenesis screen to identify genes regulating mineralized craniofacial, axial and dental development. The fact that zebrafish continuously regenerate their teeth makes them an extremely useful model to study tooth regeneration. Our goal is to identify and characterize molecular genetic signaling pathways regulating these processes, which can be manipulated via targeted gene delivery strategies. Through these efforts, we hope to eventually define methods for effective, clinically relevant bone and tooth replacement therapies in humans. Here, we describe our studies using the zebrafish model, which are proving to be useful for the identification and characterization of genes regulating mineralized tissue formation, regeneration, and homeostasis. Although preliminary at the present time, we anticipate the elucidation of novel signaling pathways regulating bone and tooth regeneration, which will eventually facilitate the repair of human skeletal and dental dysplasias.