Two MYB activators of anthocyanin biosynthesis exhibit specialized activities in petiole and fruit of diploid strawberry
Two MYB activators of anthocyanin biosynthesis exhibit specialized activities in petiole and fruit of diploid strawberry
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DOI：
10.1093/jxb/erac507
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发表时间：
2023-02-09
影响因子：
6.9
通讯作者：
Liu, Zhongchi
中科院分区：
文献类型：
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作者：
Luo, Xi;Plunkert, Madison;Liu, Zhongchi
R2R3-MYB转录因子FveMYB10是草莓红色果实花青素色素沉着的主要调控因子。Fvemyb10功能缺失突变体形成黄色果实，但叶柄中仍积累紫色花青素，表明花青素的生物合成在果实和叶柄中受到不同的调控。通过对二倍体野草莓Fragaria vesca的化学诱变，鉴定出叶柄中缺乏花青素的绿叶柄(gp)-1突变体。通过测序定位和瞬时功能分析，我们证实了致病突变位于FveMYB10样基因（FveMYB10L）中，FveMYB10和FveMYB10L分别在果实和叶柄中独立起作用。除了组织特异性调控外，FveMYB10和FveMYB10L对光质量变化的响应不同，产生不同的花青素成分，并优先激活各自组织中不同的下游花青素生物合成基因。本研究发现了一个新的花青素合成调节因子，并证明了两个具有特殊功能的同源MYB基因能够在果实和叶柄组织中对花青素的生物合成进行组织特异性调节。草莓植物通过转录因子MYB10-Like在叶柄中积累花青素。它的表达和下游靶基因与MYB10不同，MYB10是果实色素沉着的调节因子。
The R2R3-MYB transcription factor FveMYB10 is a major regulator of anthocyanin pigmentation in the red fruits of strawberry. fvemyb10 loss-of-function mutants form yellow fruits but still accumulate purple-colored anthocyanins in the petioles, suggesting that anthocyanin biosynthesis is under distinct regulation in fruits and petioles. From chemical mutagenesis in the diploid wild strawberry Fragaria vesca, we identified a green petioles (gp)-1 mutant that lacks anthocyanins in petioles. Using mapping-by-sequencing and transient functional assays, we confirmed that the causative mutation resides in a FveMYB10-Like (FveMYB10L) gene and that FveMYB10 and FveMYB10L function independently in the fruit and petiole, respectively. In addition to their tissue-specific regulation, FveMYB10 and FveMYB10L respond differently to changes in light quality, produce distinct anthocyanin compositions, and preferentially activate different downstream anthocyanin biosynthesis genes in their respective tissues. This work identifies a new regulator of anthocyanin synthesis and demonstrates that two paralogous MYB genes with specialized functions enable tissue-specific regulation of anthocyanin biosynthesis in fruit and petiole tissues.Strawberry plants accumulate anthocyanin in petioles through the action of the transcription factor MYB10-Like. Its expression and downstream target genes differ from MYB10, a regulator of pigmentation in the fruit.