Zinc-Deficient Diet Decreases Fetal Long Bone Growth Through Decreased Bone Matrix Formation in Mice
Zinc-Deficient Diet Decreases Fetal Long Bone Growth Through Decreased Bone Matrix Formation in Mice
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DOI：
10.1089/jmf.2007.0647
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发表时间：
2009-02-01
影响因子：
2.4
通讯作者：
Shin, Hong-In
中科院分区：
文献类型：
--
作者：
Kim, Jung-Tak;Baek, Sang-Heum;Shin, Hong-In
本研究以缺锌（3 mg/kg）或足锌（30 mg/kg）的ICR孕鼠为研究对象，评估了锌对胎儿发育期间骨骼发育的影响。我们还纳入了一组与缺锌组配对喂养，以控制因缺锌导致的食欲下降。采用剖宫产法取出胚胎18.5天发育中的胎儿，通过组织学分析、体重和纵向生长测量来评估骨骼发育。与锌充足组相比，缺锌组和配对喂养组母亲食物摄入量减少导致胎儿体重显著下降（P < 0.05）。然而，与缺锌组相比，配对喂养组的胎儿长度发育迟缓不那么明显，这表明母体循环中锌供应的减少可能通过骨骼发育在纵向生长中发挥作用。缺锌组胎儿发育中的胫骨骨干明显缩短，肥厚软骨细胞带宽度轻度变窄，破骨细胞数量增加，但对骨基质矿化无影响。这可能是成骨细胞活性降低和软骨细胞成熟的结果，同时破骨细胞活性增加，这表明胎儿发育期间缺锌对骨基质形成的影响大于骨基质矿化。
This study evaluated the effects of zinc on skeletal development during fetal development in pregnant ICR mice fed a zinc-deficient (3 mg/kg) or zinc-adequate (30 mg/kg) diet. We also included a group pair-fed with the zinc-deficient group to control for decreased appetite due to zinc deficiency. Developing fetuses at embryonic day 18.5 were removed by cesarean section, and the skeletal development was evaluated by histological analysis as well as by body weight and longitudinal growth measurement. Reduced maternal food intake in the zinc-deficient and pair-fed groups resulted in a marked and significant (P < .05) decrease in fetal weight compared to that of the zinc-adequate group. However, fetal length retardation in the pair-fed group was less marked than in the zinc-deficient group, suggesting that reduced supply of zinc from maternal circulation may play a role in longitudinal growth through skeletal development. The fetal developing tibia of the zinc-deficient group showed marked shortening of diaphysis and a mild narrowing of the hypertrophic chondrocyte zone width with increased osteoclast number, but there was no influence on the mineralization of bone matrix. This may be the result of reduced activation of osteoblasts and maturation of chondrocytes with increased osteoclastic activity, suggesting that zinc deficiency during the fetal development has a greater impact on the matrix formation of bone than the mineralization of bone matrix.