Sugar metabolism as related to the cyanide-mediated elimination of dormancy in apple embryos
Sugar metabolism as related to the cyanide-mediated elimination of dormancy in apple embryos
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DOI：
10.1016/s0981-9428(00)80110-2
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发表时间：
1999-07-01
影响因子：
6.5
通讯作者：
Lewak, S
中科院分区：
文献类型：
--
作者：
Bogatek, R;Côme, D;Lewak, S
从苹果休眠种子中分离出胚，CV. Antonowka）用气态HCN（1 mM）处理6 h，然后在光照下培养9d，以对照未处理的胚胎。可溶性糖进行了定量，寡糖水解酶的活性进行了测定，在轴和子叶的胚在培养过程中。HCN预处理促进胚轴萌发和寡糖水解。HCN对下部子叶蔗糖水解的影响较小。另一方面，生长，绿化和蔗糖水解在上部子叶刺激HCN预处理的水平，观察到在下部子叶。据推测，苹果种子中的内源HCN控制胚休眠的解除平行于前面描述的光和赤霉素的控制。赤霉素被证明对贮藏脂质的水解和进一步转化起作用，包括脂肪酸的β-氧化，但不起作用。看来，水解低聚糖及其催化剂是氰化物的目标。(C)爱思唯尔，巴黎。
Embryos isolated from dormant seeds of apple (Malus domestica Borb., cv. Antonowka) were treated by gaseous HCN (1 mM) for 6h and then cultured in the light for 9 d in parallel to control non-treated embryos. Soluble sugars were quantified, and oligosaccharide hydrolysing enzyme activities were determined in axes and in cotyledons of such embryos during culture. HCN pre-treatment stimulated germination and hydrolysis of oligosaccharides in embryonic axes. Hydrolysis of sucrose in the lower cotyledon tin contact with wet medium and therefore growing and greening faster) was affected by HCN to a small extent. On the other hand, growth, greening and sucrose hydrolysis in the upper cotyledon were stimulated by HCN pre-treatment to the levels observed in lower cotyledon. It is postulated that endogenous HCN in apple seeds controls the removal of embryonic dormancy in parallel to the earlier described control by light and gibberellin. Gibberellin was demonstrated to act on the hydrolysis and further transformations of storage lipids, including gluconeogenesis, but not (beta-oxidation of fatty acids. It appears that hydrolysis of oligosaccharides and their catabolism is the target for cyanide. (C) Elsevier, Paris.