Spherical nanoparticle supported lipid bilayers for the structural study of membrane geometry-sensitive molecules.
Spherical nanoparticle supported lipid bilayers for the structural study of membrane geometry-sensitive molecules.
复制标题
DOI：
10.1021/jacs.5b08303
复制
发表时间：
2015-11-11
影响因子：
15
通讯作者：
Tian F
中科院分区：
文献类型：
--
作者：
Fu R;Gill RL Jr;Kim EY;Briley NE;Tyndall ER;Xu J;Li C;Ramamurthi KS;Flanagan JM;Tian F
许多基本的细胞过程，包括内吞作用和囊泡运输需要改变膜的几何形状。这些变化通常由可以感知和/或诱导膜弯曲的蛋白质介导。使用球形纳米粒子支持的脂质双层（SSLB），我们表征了SpoVM，细菌发育因子，如何通过魔角旋转固态NMR与不同弯曲的膜相互作用。我们的结果表明，SSLB是膜几何敏感分子结构和拓扑研究的有效系统。
Many essential cellular processes including endocytosis and vesicle trafficking require alteration of membrane geometry. These changes are usually mediated by proteins that can sense and/or induce membrane curvature. Using spherical nanoparticle supported lipid bilayers (SSLBs), we characterize how SpoVM, a bacterial development factor, interacts with differently curved membranes by magic angle spinning solid-state NMR. Our results demonstrate that SSLBs are an effective system for structural and topological studies of membrane geometry-sensitive molecules.