Exclusion of melanocortin-1 receptor (Mc1r) and Agouti as candidates for dominant black in dogs
Exclusion of melanocortin-1 receptor (Mc1r) and Agouti as candidates for dominant black in dogs
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DOI：
10.1093/jhered/esg016
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发表时间：
2003-01-01
影响因子：
3.1
通讯作者：
Barsh, GS
中科院分区：
文献类型：
--
作者：
Kerns, JA;Olivier, M;Barsh, GS
家犬表现出多种毛色，不同品种之间存在很大的差异。人们对狗色素沉着的分子生物学知之甚少。目前的理解主要基于传统的育种实验，在某些情况下，这些实验表明与其他哺乳动物报道的遗传相互作用不同。我们研究了显性黑色的分子遗传学，显性黑色是黑色拉布拉多猎犬或纽芬兰猎犬的均匀毛色特征，被认为是由 melanocortin-1 受体基因 (Mc1r) 的变异或 Agouti 基因 (A) 的变异引起的。我们鉴定了 Mc1r 内的几个编码多态性和几个与 A 密切相关的简单序列重复多态性，并检查了它们在拉布拉多猎犬 X 灰狗杂交中的遗传，该杂交分离了占主导地位的黑色。在携带显性黑色的动物中没有一致地发现单一的 Mc1r 等位基因，并且 Mc1r 和 A 都不与显性黑色共分离。这些结果完善了我们对哺乳动物毛色遗传的理解，并表明狗的黑色毛色占主导地位是由以前未涉及色素类型转换的基因引起的。
The domestic dog exhibits a variety of coat colors that encompass a wide range of variation among different breeds. Very little is known about the molecular biology of dog pigmentation; current understanding is based mostly on traditional breeding experiments, which in some cases have suggested genetic interactions that are different from those reported in other mammals. We have examined the molecular genetics of dominant black, a uniform coat color characteristic of black Labrador retrievers or Newfoundlands that has been proposed to be caused by either variation in the melanocortin-1 receptor gene (Mc1r) or by variation in the Agouti gene (A). We identified several coding polymorphisms within Mc1r and several simple sequence repeat polymorphisms closely linked to A, and examined their inheritance in a Labrador retriever X greyhound cross that segregates dominant black. No single Mc1r allele was found consistently in animals carrying dominant black, and neither Mc1r nor A cosegregated with dominant black. These results refine our understanding of mammalian coat color inheritance and suggest that dominant black coat color in dogs is caused by a gene not previously implicated in pigment type switching.