MitoTALENs: A Method for Targeted Gene Disruption in Plant Mitochondrial Genomes
MitoTALENs: A Method for Targeted Gene Disruption in Plant Mitochondrial Genomes
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MitoTALENs：植物线粒体基因组中靶向基因破坏的方法
DOI：
10.1007/978-1-0716-1653-6_22
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发表时间：
2021
影响因子：
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通讯作者：
Arimura Shin-ichi
中科院分区：
文献类型：
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作者：
Kang Byung-Ho;Anderson Charles T;Arimura Shin-ichi;Bayer Emmanuelle;Bezanilla Magdalena;Botella Miguel A;Brandizzi Federica;Burch-Smith Tessa M;Chapman Kent D;D?nser Kai;Gu Yangnan;Jaillais Yvon;Kirchhoff Helmut;Otegui Marisa S;Rosado Abel;Tang Yu;Kleine-;Arimura Shin-ichi
植物线粒体基因组的修饰仍然是一项艰巨的任务，特别是在多细胞植物中。具有线粒体定位信号的转录激活因子样效应核酸酶（mitoTALENs）可以从植物线粒体基因组中剪切出所需的序列。虽然mitoTALENs的载体构建较为复杂，但其修饰效率高，足以实现多拷贝线粒体基因组的同质化。在这里，我描述了如何设计mitoTALENs来选择一个目标，构建一个载体，并选择线粒体转化子。
Modification of plant mitochondrial genomes is still a difficult task, especially in multicellular plants. Transcription activator-like effector nucleases with a mitochondrial localization signal (mitoTALENs) can cut out a desired sequence from the mitochondrial genome in plants. Although vector construction of mitoTALENs is complicated, the modification efficiency is high enough to achieve homoplasmy of multicopy mitochondrial genomes. Here I describe how to design mitoTALENs to select a target, construct a vector, and select the mitochondrial transformants.