Photooxidative Polymerization of Pyrrole from Photosystem I Proteins
Photooxidative Polymerization of Pyrrole from Photosystem I Proteins
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DOI：
10.1021/acsapm.2c01404
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发表时间：
2022-10-14
影响因子：
5
通讯作者：
Jennings, G. Kane
中科院分区：
文献类型：
--
作者：
Passantino, Joshua M.;Williams, Alessia M.;Jennings, G. Kane
光系统I（PSI）蛋白复合物由于其强大的光电化学性质而被越来越多地利用。与FB反应位点的还原能力相比，蛋白质的P700反应位点的氧化能力研究较少。如本文所示，P700的氧化电位足够稳健以引发氧化还原单体吡咯的氧化聚合。该手稿报道了第一种光活性和导电蛋白质-聚合物复合物，其使用提取的未修饰的PSI蛋白质来进行聚合过程。聚合技术包括将单体和掺杂剂添加到PSI溶液中，并用模拟阳光照射样品。通过红外光谱、目视观察、热重分析、接触角测量和电导率测量的组合证实了PSI中吡咯的成功聚合。蛋白质和聚合物之间的稳定复合物由SDS-PAGE指示，并且进一步由在电流和电势上与单独的PSI相反的光电化学性质支持。
The photosystem I (PSI) protein complex is increasingly being utilized because of its robust photoelectrochem-ical properties. The oxidative ability of the P700 reaction site of the protein is less studied than the reductive capabilities of the FB reaction site. As shown herein, the oxidation potential of P700 is robust enough to initiate an oxidative polymerization of the redox monomer, pyrrole. This manuscript reports the first photoactive and conductive protein-polymer composite using extracted, unmodified PSI protein to perform the polymerization process. The polymerization technique consists of adding a monomer and a dopant to a solution of PSI and illuminating the sample with simulated sunlight. Successful polymerization of pyrrole from PSI is confirmed by the combination of infrared spectroscopy, visual observation, thermogravimetric analysis, contact angle measurements, and conductivity measurements. A stable composite between the protein and polymer is indicated by SDS-PAGE and is further supported by photoelectrochemical properties that are opposite in current and potential from those of PSI alone.