The prevalence of Arcobacter spp. on chicken carcasses sold in retail markets in Turkey, and identification of the isolates using SDS-PAGE
The prevalence of Arcobacter spp. on chicken carcasses sold in retail markets in Turkey, and identification of the isolates using SDS-PAGE
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DOI：
10.1016/s0168-1605(02)00163-0
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发表时间：
2003-02-25
影响因子：
5.4
通讯作者：
Vandamme, P
中科院分区：
文献类型：
--
作者：
Atabay, HI;Aydin, F;Vandamme, P
在本研究中，研究了弧菌的流行情况。对土耳其不同零售市场出售的鸡尸进行了调查。用不同的表型和分子检测方法对菌株进行了鉴定。在非选择性血液琼脂上放置0.45um孔径的膜过滤器，经氧化弧菌增菌液(AEB)浓缩后，使用膜过滤技术对75只鸡身体(新鲜44只，冷冻31只)进行检测。利用全细胞蛋白的SDS-PAGE和最近发展的多重PCR方法进行物种水平的鉴定。经鉴定，所有菌株均为巴氏弧菌。在所检验的44只新鲜鸡尸中，42只(95%)对布氏假单胞菌呈阳性反应。在被检查的31具冷冻身体中，也有7具(23%)被发现。(C)2002 Elsevier Science B.V.保留所有权利。
In this study, the prevalence of Arcobacter spp. on chicken carcasses sold in various retail markets in Turkey was investigated. The isolates were characterized and identified using various phenotypic and molecular tests. The membrane filtration technique employing 0.45-mum pore size membrane filters laid onto a nonselective blood agar was used after enrichment in Oxoid Arcobacter Enrichment Broth (AEB) to examine a total of 75 chicken carcasses (44 fresh and 31 frozen). Species level identification was performed using SDS-PAGE of whole-cell proteins and a recently developed multiplex-PCR assay. All isolates were identified as Arcobacter butzleri. Of the 44 fresh chicken carcasses examined, 42 (95%) were positive for A. butzleri. A. butzleri was also recovered from seven (23%) of the 31 frozen carcasses examined. (C) 2002 Elsevier Science B.V. All rights reserved.