Detection of tau phosphorylated at threonine 231 in cerebrospinal fluid of Alzheimer's disease patients
Detection of tau phosphorylated at threonine 231 in cerebrospinal fluid of Alzheimer's disease patients
复制标题
DOI：
10.1016/s0304-3940(00)01178-2
复制
发表时间：
2000-06-30
影响因子：
2.5
通讯作者：
Hampel, H
中科院分区：
文献类型：
--
作者：
Kohnken, R;Buerger, K;Hampel, H
描述了一种新的夹心ELISA，其对在苏氨酸231处磷酸化的tau显示出特异性，并且相对于其他痴呆症，优先与阿尔茨海默病（AD）脑提取物反应。该方法用于分析58个死前脑脊液样本。27个AD样本中的23个（85%灵敏度）产生的信号大于截止值，而31个非AD样本中只有一个（97%特异性）更大。这表明，用这种夹心ELISA检测脑脊液中的磷酸化tau可能有助于AD的诊断。(C)2000爱思唯尔科学爱尔兰有限公司保留所有权利。
A new sandwich ELISA is described which shows specificity for tau phosphorylated at threonine 231 and preferentially reacts with Alzheimer's disease (AD) brain extracts relative to other dementias. This assay was used to analyze 58 antemortem cerebrospinal fluid samples. Twenty-three of 27 AD samples (85% sensitivity) yielded signals greater than the cutoff, while only one of 31 non-AD samples (97% specificity) were greater. This indicates that detection of phosphotau in cerebrospinal fluid with this sandwich ELISA could prove useful in the diagnosis of AD. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.