Lysosomal apparatus in uterine muscle: effects of estrogen and of ovariectomy.
Lysosomal apparatus in uterine muscle: effects of estrogen and of ovariectomy.
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子宫肌肉中的溶酶体装置：雌激素和卵巢切除术的影响。
DOI：
10.1095/biolreprod23.4.867
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发表时间：
1980
影响因子：
3.6
通讯作者：
Sloane,BF
中科院分区：
文献类型：
--
作者：
Sloane,BF
在非妊娠大鼠的子宫肌肉中，溶酶体酶的比活性随着卵巢激素状态的变化而变化，并且与肝脏中发现的相似。然而，在之前的形态学研究中，在骑行大鼠的子宫肌肉中并未发现溶酶体。在这项研究中，我们检查了发情期间、卵巢切除后以及随后注射雌激素和/或黄体酮后的大鼠子宫肌肉的超微结构，即在作者在早期生化研究中使用的相同实验条件下（Sloane 和 Bird，1977）。在雌激素支配下的子宫肌细胞中，经常观察到溶酶体致密体。这些细胞还具有广泛的粗面内质网（RER）和高尔基体以及许多液泡和残留体。卵巢切除后 1 天内，可以观察到包被囊泡和初级溶酶体从高尔基体或 GERL（高尔基体和 RER 之间的小管区域，溶酶体从其中萌芽）出芽。卵巢切除术后 3 周，唯一存在的溶酶体结构是一些残留的身体。子宫肌细胞中溶酶体数量的变化与溶酶体酶活性的变化相关，但溶酶体的数量似乎不足以解释酶活性，这表明子宫肌细胞中的溶酶体酶可能储存在RER、高尔基体或GERL中。
In uterine muscle from nongravid rats, the specific activities of lysosomal enzymes change with ovarian hormonal state and approximate those found in liver. However, in previous morphological studies lysosomes were not found in uterine muscle from cycling rats. In this study we examined the ultrastructure of rat uterine muscle during estrus, after ovariectomy, and after subsequent injection of estrogen and/or progesterone, i.e., under the same experimental conditions used by the author in an earlier biochemical study (Sloane and Bird, 1977). In uterine muscle cells under estrogen domination, lysosomal dense bodies were routinely observed. These cells also had an extensive rough endoplasmic reticulum (RER) and Golgi apparatus as well as many vacuoles and residual bodies. Within 1 day after ovariectomy, coated vesicles and primary lysosomes could be observed budding from the Golgi or GERL (region of tubules between the Golgi and RER from which lysosomes bud). At 3 weeks postovariectomy, the only lysosomal structures present were a few residual bodies. Changes in the number of lysosomes in uterine muscle cells correlated with changes in lysosomal enzyme activities, yet the number of lysosomes did not seem sufficient to account for the enzyme activity, suggesting that lysosomal enzymes in uterine muscle may be stored in the RER, Golgi, or GERL.