Dual in vivo magnetic resonance evaluation of magnetically labeled mouse embryonic stem cells and cardiac function at 1.5 T
Dual in vivo magnetic resonance evaluation of magnetically labeled mouse embryonic stem cells and cardiac function at 1.5 T
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DOI：
10.1002/mrm.20702
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发表时间：
2006-01-01
影响因子：
3.3
通讯作者：
Yang, PC
中科院分区：
文献类型：
--
作者：
Arai, T;Kofidis, T;Yang, PC
细胞疗法已被证明具有恢复受损心肌的潜力。一种可靠的体内成像方法来定位移植细胞并监测其恢复效果，将能够对这种治疗方式进行系统研究。证明了磁标记小鼠胚胎干细胞（mESC）成像的双重MRI能力及其对急性心肌梗死小鼠模型心脏功能的恢复作用。使用1.5 T临床扫描仪在4周内对移植的mESC进行系列体内MR检测并监测mESC治疗的心肌。在4周的持续时间内，mESC治疗的心肌表现出左心室（LV）射血分数的持续改善和LV质量的保护。此外，磁标记mESC对心功能的恢复作用无显著差异。因此，体内MRI能够同时检测移植的mESC及其对损伤心肌的治疗效果。
Cell therapy has demonstrated the potential to restore injured myocardium. A reliable in vivo imaging method to localize transplanted cells and monitor their restorative effects will enable a systematic investigation of this therapeutic modality. The dual MRI capability of imaging both magnetically labeled mouse embryonic stem cells (mESC) and their restorative effects on cardiac function in a murine model of acute myocardial infarction is demonstrated. Serial in vivo MR detection of transplanted mESC and monitoring of the mESC-treated myocardium was conducted over a 4-week period using a 1.5 T clinical scanner. During the 4-week duration, the mESC-treated myocardium demonstrated sustained improvement of the left ventricular (LV) ejection fraction and conservation of LV mass. Furthermore, no significant difference of their restorative effects on the cardiac function was created by the magnetic labeling of mESC. Thus, in vivo MRI enables simultaneous detection of transplanted mESC and their therapeutic effect on the injured myocardium.