Evolution in Candida albicans Populations During a Single Passage Through a Mouse Host
Evolution in Candida albicans Populations During a Single Passage Through a Mouse Host
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DOI：
10.1534/genetics.109.103325
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发表时间：
2009-07-01
期刊：
影响因子：
3.3
通讯作者：
May, Georgiana
中科院分区：
文献类型：
--
作者：
Forche, Anja;Magee, P. T.;May, Georgiana
机会致病真核病原体在宿主中生长过程中产生基因型和表型变异的机制和速率尚不清楚。我们评估了白色念珠菌，所有人类机会致病真菌病原体，在通过小鼠宿主（体内）和在液体培养（体外）繁殖过程中的全基因组遗传和表型进化。我们发现，相对于体外生长的种群，体内传代的种群的种群增长较慢，染色体水平的遗传变异率较高。有趣的是，在两种生长环境中，基因组中长距离杂合性丢失（洛）和染色体重排事件的分布不同，而短距离杂合性丢失（LOH）和染色体重排事件的发生率不同。体内和体外群体的洛范围相当。此外，对于患病的体内群体，存在细胞的正相关性，表明遗传改变和菌落生长和形态的变化。对于体外种群，未检测到生长表型的变化。总之，我们的研究结果表明，通过它活的主机导致更慢的增长和更高的基因组和表型变异率相比，在体外人口。结果表明，种群增长和全基因组重排的动态有助于维持一个寄生性和机会主义的生活史。白色念珠菌
The mechanisms and rates by which genotypic and phenotypic variation is generated in opportunistic, eukaryotic pathogens during growth in hosts are not well understood. We evaluated genomewide genetic and phenotypic evolution in Candida albicans, all opportunistic fungal pathogen of humans, during passage through a mouse host (in vivo) and during propagation in liquid culture (in vitro). We found slower population growth and higher rates of chromosome-level genetic variation in populations passaged in vivo relative to those grown in vitro. Interestingly, the distribution of long-range loss of heterozygosity (LOH) and chromosome rearrangement events across the genome differed for the two growth environments, while rates of short.-range LOH were comparable for in vivo and in vitro populations. Further, for the ill vivo populations, there was a positive correlation of cells demonstrating genetic alterations and variation in colony growth and morphology. For in vitro populations, no variation in growth phenotypes was detected. Together, our results demonstrate that passage through it living host leads to slower growth and higher rates of genomic and phenotypic variation compared to in vitro populations. Results suggest that the dynamics of population growth and genomewide rearrangement contribute to the maintenance of a commensal and opportunistic life history of C. albicans.