CLONING AND EXPRESSION IN ESCHERICHIA-COLI OF THE GENE ENCODING A NOVEL L-2,4-DIAMINOBUTYRATE DECARBOXYLASE OF ACINETOBACTER-BAUMANNII
CLONING AND EXPRESSION IN ESCHERICHIA-COLI OF THE GENE ENCODING A NOVEL L-2,4-DIAMINOBUTYRATE DECARBOXYLASE OF ACINETOBACTER-BAUMANNII
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DOI：
10.1111/j.1574-6968.1994.tb07288.x
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发表时间：
1994-12-01
影响因子：
2.1
通讯作者：
YAMAMOTO, S
中科院分区：
文献类型：
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作者：
IKAI, H;YAMAMOTO, S
从鲍氏不动杆菌ATCC 19606中克隆了编码L-2，4-二氨基丁酸脱羧酶（DABA DC）的基因。该基因显然受其自身启动子的控制。有趣的是，携带该克隆的宿主也产生可观量的1，3-二氨基丙烷。限制性酶切图谱和随后的克隆插入物的亚克隆将DABA DC基因定位在2.45-kb SphI/EcoRI片段内。为了内源性生产DAP，进一步需要DABA DC基因下游的1.75-kb EcoRI/Pst I区域。Southern印迹杂交显示，在其他不动杆菌属物种之间的DABA DC基因的一些异质性。
The gene encoding L-2,4-diaminobutyrate decarboxylase (DABA DC) was cloned from Acinetobacter baumannii ATCC 19606. The gene was evidently under the control of its own promoter. Interestingly, the host carrying this clone also produced an appreciable amount of 1,3-diaminopropane. Restriction mapping and subsequent subcloning of the cloned insert localized the DABA DC gene within a 2.45-kb SphI/EcoRI fragment. For endogenous production of DAP, a 1.75-kb EcoRI/PstI region downstream from the DABA DC gene was further required. Southern blot hybridization revealed some heterogeneity in the DABA DC genes among other Acinetobacter species.