Time course of cigarette smoke-induced pulmonary inflammation in mice
Time course of cigarette smoke-induced pulmonary inflammation in mice
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DOI：
10.1183/09031936.05.00095204
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发表时间：
2005-08-01
影响因子：
24.3
通讯作者：
Pauwels, RA
中科院分区：
文献类型：
--
作者：
D'hulst, AI;Vermaelen, KY;Pauwels, RA
气道和肺实质的炎症在慢性阻塞性肺病的发病机制中起着重要作用。在本研究中，使用烟草烟雾诱发的肺气肿小鼠模型来研究气道和肺部炎症反应的时间过程，特别关注肺树突状细胞（DC）群体。各组小鼠暴露于香烟烟雾或控制空气长达24周。为了响应香烟烟雾，炎症细胞（即中性粒细胞、巨噬细胞和淋巴细胞）逐渐在小鼠气道和肺实质中积累。此外，在24周时，在暴露于香烟的小鼠的气道（增加10倍）和肺实质（增加1.5倍）中观察到DC的明显浸润。对暴露于烟雾的小鼠的支气管肺泡灌洗（BAL）DC进行流式细胞分析显示，与对照组相比，主要组织相容性复合物II分子和共刺激分子CD40和CD86上调。 暴露在空气中的小鼠的 BAL DC。肺组织学的形态计量学分析表明，在烟雾暴露 24 周后，平均线性截距和肺泡壁破坏显着增加。 总之，仔细描述了暴露于香烟烟雾的小鼠的支气管肺泡灌洗液和肺室中炎症和树突细胞变化的时间过程。
Inflammation of the airways and lung parenchyma plays a major role in the pathogenesis of chronic obstructive pulmonary disease. In the present study a murine model of tobacco smoke-induced emphysema was used to investigate the time course of airway and pulmonary inflammatory response, with a special emphasis on pulmonary dendritic cell (DC) populations.Groups of mice were exposed to either cigarette smoke or to control air for up to 24 weeks. In response to cigarette smoke, inflammatory cells (i.e. neutrophils, macrophages and lymphocytes) progressively accumulated both in the airways and lung parenchyma of mice. Furthermore, a clear infiltration of DCs was observed in airways (10-fold increase) and lung parenchyma (1.5-fold increase) of cigarette-exposed mice at 24 weeks.Flow cytometric analysis of bronchoalveolar lavage (BAL) DCs of smoke-exposed mice showed upregulation of major histocompatability complex II molecules and costimulatory molecules CD40 and CD86, compared with BAL DCs of air-exposed mice. Morphometric analysis of lung histology demonstrated a significant increase in mean linear intercept and alveolar wall destruction after 24 weeks of smoke exposure.In conclusion, the time course of the changes in inflammatory and dendritic cells in both bronchoalveolar lavage and the pulmonary compartment of cigarette smoke-exposed mice was carefully characterised.