Saponin-induced release of single cells from filaments and rhizoid differentiation in Spirogyra
Saponin-induced release of single cells from filaments and rhizoid differentiation in Spirogyra
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DOI：
10.1007/s10265-004-0177-z
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发表时间：
2004-12-01
影响因子：
2.8
通讯作者：
Shimmen, T
中科院分区：
文献类型：
--
作者：
Ikegaya, H;Yamada, S;Shimmen, T
一些生活在溪流中的水绵可以通过从丝状体的末端细胞分化出假根而锚在基质上。在光照条件下，类胡萝卜素的分化发生，但在黑暗条件下则不发生。将具有假根分化能力的水绵丝状体在含0.1%皂甙的培养基中培养，末端细胞一个接一个地释放出来，形成单细胞。去除培养液中的皂甙后，单细胞可有效地分化为假根。本研究建立的单细胞系统适用于水绵假根分化过程中基因表达的分析。
Some species of Spirogyra living in streams can anchor to the substratum by differentiating a rhizoid from a terminal cell of a filaments. Rhizoid differentiation occurs in the light but not in the dark. When a filament of Spirogyra sp. competent for rhizoid differentiation was incubated in a medium containing 0.1% saponin, terminal cells were released one by one, forming single cells. Single cells effectively differentiated to be rhizoids when saponin in the incubation medium was removed. The single-cell system developed in the present study seems suitable for analysis of gene expression during rhizoid differentiation of Spirogyra.