HISTONE GENES ARE LOCATED AT THE SPHERE LOCI OF XENOPUS LAMPBRUSH CHROMOSOMES
HISTONE GENES ARE LOCATED AT THE SPHERE LOCI OF XENOPUS LAMPBRUSH CHROMOSOMES
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DOI：
10.1007/bf00365156
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发表时间：
1991-12-01
期刊：
影响因子：
1.6
通讯作者：
MURPHY, C
中科院分区：
文献类型：
--
作者：
CALLAN, HG;GALL, JG;MURPHY, C
在无尾类非洲爪蟾中，正如先前在有尾目两栖纲的几个物种中所证明的那样，组蛋白基因位于灯刷染色体的球组织者位点。 它们被定位通过原位杂交的H-3-标记的组蛋白H4反义cRNA探针应用于灯刷制剂中的转录RNA已被保留，同样的制剂中的转录本是不存在的，但其DNA已变性杂交前。 在非洲爪蟾中，组蛋白基因与附着在灯刷染色体上的球体密切相关，但在萌发囊泡中游离的球体中却不存在。 无尾目在几亿年前从尾目分离，所以球状组织者/组蛋白基因的关联是非常古老的。 这表明这种关联具有功能性意义，尽管它尚未被发现。
In the anuran Xenopus, as has been demonstrated previously in several species of urodele Amphibia, histone genes lie at the sphere organizer loci of the lampbrush chromosomes. They were located by in situ hybridization of a H-3-labelled histone H4 anti-sense cRNA probe applied to lampbrush preparations in which transcript RNA had been retained, and likewise to preparations in which transcripts were absent but whose DNA had been denatured prior to hybridization. In Xenopus the histone genes lie in intimate association with the spheres that are attached to the lampbrush chromosomes, but they are absent from spheres that lie free in the germinal vesicle. The Anura separated from the Urodela several hundred million years ago, so the sphere organizer/histone gene association is of great antiquity. This suggests that the association has a functional significance, though it is one that has yet to be discovered.