Melanin-concentrating hormone: unique peptide neuronal system in the rat brain and pituitary gland.
Melanin-concentrating hormone: unique peptide neuronal system in the rat brain and pituitary gland.
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黑色素浓缩激素：大鼠大脑和垂体中独特的肽神经元系统。
DOI：
10.1073/pnas.83.5.1528
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发表时间：
1986
影响因子：
11.1
通讯作者：
Jacobowitz,DM
中科院分区：
文献类型：
--
作者：
Zamir,N;Skofitsch,G;Bannon,MJ;Jacobowitz,DM
用鲑鱼黑素浓缩激素(MCH)抗体进行免疫组织化学和放射免疫分析，在大鼠中枢神经系统中发现了一种独特的神经系统。MCH样免疫反应阳性(MCH-LI)胞体局限于下丘脑。MCH-LI纤维分布于全脑，但在下丘脑、中脑和脑桥-髓质区最为常见。在下丘脑内侧前脑束(MFB)、下丘脑后核和斜角带核检测到较高浓度的MCH-LI。大鼠脑MFB提取物的反相高效液相色谱分析表明，大鼠的MCH样肽与鲑鱼的MCH具有不同的保留时间。渗透刺激(2%氯化钠作为饮用水，持续120小时)引起下丘脑外侧叶和神经中间叶MCH-LI浓度显著增加。目前的研究证实了大鼠脑内存在MCH样肽。MCH-LI神经元系统处于很好的位置，可以协调复杂的功能，如调节水分摄入。
A unique neuronal system was detected in the rat central nervous system by immunohistochemistry and radioimmunoassay with antibodies to salmon melanin-concentrating hormone (MCH). MCH-like immunoreactive (MCH-LI) cell bodies were confined to the hypothalamus. MCH-LI fibers were found throughout the brain but were most prevalent in hypothalamus, mesencephalon, and pons-medulla regions. High concentrations of MCH-LI were measured in the hypothalamic medial forebrain bundle (MFB), posterior hypothalamic nucleus, and nucleus of the diagonal band. Reversed-phase high-performance liquid chromatography of MFB extracts from rat brain indicate that MCH-like peptide from the rat has a different retention time than that of the salmon MCH. An osmotic stimulus (2% NaCl as drinking water for 120 hr) caused a marked increase in MCH-LI concentrations in the lateral hypothalamus and neurointermediate lobe. The present studies establish the presence of MCH-like peptide in the rat brain. The MCH-LI neuronal system is well situated to coordinate complex functions such as regulation of water intake.