Additional far-red light promotes adventitious rooting of double-root-cutting grafted watermelon seedlings
Additional far-red light promotes adventitious rooting of double-root-cutting grafted watermelon seedlings
复制标题
附加远红光促进双根扦插嫁接西瓜苗不定根
DOI：
10.1016/j.hpj.2022.11.012
复制
发表时间：
2024-11-01
影响因子：
5.7
通讯作者：
Cao，Kai
中科院分区：
文献类型：
--
作者：
Wu，Xue;Zhang，Xiaoyan;Cao，Kai
根再生是影响嫁接愈合率和双截根嫁接成活率的重要因素。迄今为止，关于如何提高嫁接西瓜幼苗砧木的再生能力的研究还很少。在本研究中，测定了不同光照处理对根再生的影响。这表明，添加远红光（Fr）可以显著促进砧木的根形成。此外，转录组分析结果显示，Fr对植物激素通路和生长素相关基因的诱导作用较大，特别是对生长素反应蛋白（包括cmiaa11、CmIAA17和cmaux28）、生长素小RNA基因（包括cmsaur20和cmsaur50）和生长素外排转运蛋白（CmPIN3）的诱导作用。此外，光敏色素相互作用因子（PIFs）如cmpif1、cmpif3和cmpif7的表达在Fr的作用下显著增加。这些基因可能在Fr的作用下共同激活生长素相关通路。HPLC-MS/MS分析结果表明，Fr对不定根中不同类型生长素的浓度有显著影响，并且通过酸性品红染色实验证实，Fr处理的砧木根生长较好，嫁接体的血管运输活性较强。综上所述，Fr可能通过调控生长素相关基因和PIF基因的转录水平，诱导砧木AR的形成，并与生长素积累有关。本研究结果为缩短西瓜嫁接愈合期、提高西瓜嫁接苗质量提供了一条切实可行的途径，为加快产业化种苗生产提供了理论依据。
Root regeneration is an important factor influencing the healing rate of graft union and the survival of double-root-cutting grafting. To date, little information is available on how to enhance root regeneration of rootstock in grafted watermelon (Citrullus lanatus) seedlings. In this study, the effects of different light treatments on root regeneration were determined. This revealed that addition of far-red light (Fr) could significantly expedite root formation in the rootstock. Moreover, the results of transcriptome analysis revealed that plant hormone pathway and auxin-related genes were greatly induced by Fr, especially for auxin-response proteins (includingCmIAA11,CmIAA17,andCmAUX28), Small auxin-up RNA genes (includingCmSAUR20andCmSAUR50) and the auxin efflux transporter (CmPIN3). In addition, the expression of Phytochrome Interacting Factor (PIFs), such asCmPIF1,CmPIF3andCmPIF7, was remarkably increased by Fr. These genes may act together to activate auxin-related pathways under Fr treatment. Based on the results of HPLC-MS/MS analysis, the concentrations of different auxin-types in adventitious root were significantly influenced by Fr. Furthermore, the better growth of rootstock root displayed superior vasculature transport activity of the graft union with Fr treatment, which was determined by the acid magenta dyeing experiment. Therefore, all the results suggested that Fr could induce AR formation in rootstocks, which may be associated with the auxin accumulation by regulating the transcriptional level of auxin-related and PIF genes. The findings of this study demonstrated a practicable way to shorten the healing period of graftings and improve the quality of grafted watermelon seedlings, which will provide a theoretical basis for the speeding development of industrialized seedlings production.