Identification and characterization of a mosquito pupicidal metabolite of a Bacillus subtilis subsp subtilis strain
Identification and characterization of a mosquito pupicidal metabolite of a Bacillus subtilis subsp subtilis strain
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DOI：
10.1007/s00253-010-2449-y
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发表时间：
2010-05-01
影响因子：
5
通讯作者：
Paily, K. P.
中科院分区：
文献类型：
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作者：
Geetha, I.;Manonmani, A. M.;Paily, K. P.
枯草芽孢杆菌亚种菌株的培养上清液。从印度安达曼和尼科巴群岛的红树林中分离出的枯草芽孢杆菌被发现可以杀死蚊子的幼虫和蛹期。该细菌培养物上清液的氯仿提取物在1μg/ml的LC50剂量下显示出杀螨作用。该菌株产生的杀蚊代谢物通过凝胶渗透色谱法纯化。对纯化的级分进行傅里叶变换红外（FTIR）光谱和基质辅助激光解吸电离飞行时间（MALDI-TOF）质谱分析。活性组分/CHCl3 残基的 FTIR 光谱显示出肽的强谱带特征。样品的 MALDI-TOF 光谱显示在 m/z 值 1,030.6、1,046.7、1,044.6、1,060.5、1,058.6、1,058.7 和 1,074.6 处有很好分辨的峰组。结果表明产生了不同亚型的表面活性素，范围为 C13-C15。此外，负责产生表面活性素的sfp基因被扩增并测序。总之，本研究表明，枯草芽孢杆菌亚种产生的杀蚊蛹代谢物。枯草芽孢杆菌是环状脂肽，表面活性素。讨论了表面活性素对蚊蛹的作用方式。这是关于枯草芽孢杆菌亚种产生的表面活性素的杀蚊活性的第一份报告。枯草芽孢杆菌。
The culture supernatant of a strain of Bacillus subtilis subsp. subtilis isolated from mangrove forests of Andaman and Nicobar islands, India was found to kill larval and pupal stages of mosquitoes. A chloroform extract of the culture supernatant of the bacterium showed pupicidal effects at an LC50 dose of 1 A mu g/ml. The mosquitocidal metabolite(s) produced by this strain were purified by gel permeation chromatography. The purified fraction was subjected to Fourier transform infrared (FTIR) spectroscopy and Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry. The FTIR spectrum of active fraction/CHCl3 residue showed strong band characteristic of peptides. MALDI-TOF spectrum of the sample showed well-resolved group of peaks at m/z values 1,030.6, 1,046.7, 1,044.6, 1,060.5, 1,058.6, 1,058.7, and 1,074.6. The results indicated production of different isoforms of surfactin, ranging from C13-C15. Further, the sfp gene responsible for the production of surfactin was amplified and sequenced. In conclusion, this study showed that the mosquito pupicidal metabolite(s), produced by B. subtilis subsp. subtilis is the cyclic lipopeptide, surfactin. The mode of action of surfactin on pupae of mosquitoes is discussed. This is the first report on the mosquito pupicidal activity of surfactin produced by B. subtilis subsp. subtilis.