Detection of typical and atypical enteropathogenic Escherichia coli (EPEC) in Iranian children with and without diarrhoea
Detection of typical and atypical enteropathogenic Escherichia coli (EPEC) in Iranian children with and without diarrhoea
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DOI：
10.1099/jmm.0.46539-0
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发表时间：
2006-09-01
影响因子：
3
通讯作者：
Aslani, M. Mehdi
中科院分区：
文献类型：
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作者：
Alikhani, M. Yousef;Mirsalehian, Akbar;Aslani, M. Mehdi
本研究旨在调查典型和非典型肠致病性大肠杆菌（EPEC）在伊朗10岁以下儿童中引起感染性腹泻的原因。在夏季，对247例腹泻患儿和1108例无症状患儿标本进行了EPEC和其他细菌病原体的分析，在140例腹泻患儿中发现了潜在的肠道病原体（56例（7%））。EPEC是最常见的病原体（111例），其次是产志贺毒素大肠杆菌（113例）、志贺菌（9例）、沙门氏菌（6例）和气单胞菌（1例）。用PCR方法检测EPEC分离株中eaeA、bfpA和stx基因的存在。EPEC分离株分为典型(eaeA（+) bfpA(+)）和非典型(eaeA（+) bfpA(-)）。典型EPEC 35例（11例）。8%)，相比之下8%（8%）。4%) （P < 0.05）。从23例（9例）中分离出非典型EPEC菌株。3%)，而13%（11%）。2%)，差异有统计学意义（P < 0.05）。总之，数据表明典型和非典型EPEC是伊朗儿童腹泻的重要原因。
The present study was performed to investigate the contribution of typical and atypical enteropathogenic Escherichia coli (EPEC) as a cause of infectious diarrhoea among children less than 10 years old in Iran. During the summer months, 247 specimens from children with diarrhoea and 1108 from asymptomatic children were analysed for the presence of EPEC and other bacterial pathogens, Potential enteric pathogens were identified in 140 cases of children with diarrhoea (56(.)7%). EPEC was the most frequently identified agent (111 cases), followed by Shiga toxin-producing E coli (113), Shigella (9), Salmonella (6) and Aeromonas sp. (1). EPEC isolates were examined for the presence of eaeA, bfpA and stx genes by PCR. EPEC isolates were classified as typical (eaeA(+) bfpA(+)) or atypical (eaeA(+) bfpA(-)). Typical EPEC was diagnosed in 35 cases (11(.)8%), compared with 8(0(.)4%) in the asymptomatic group (P < 0(.)05). Atypical EPEC strains were isolated from 23 cases (9(.)3%), compared with 13(11(.)2%) of the healthy control group (P < 0(.)05). In conclusion, the data suggest that typical and atypical EPEC are an important cause of diarrhoea in Iranian children.