Cyhalofop-butyl has the potential to induce developmental toxicity, oxidative stress and apoptosis in early life stage of zebrafish (Danio rerio).
Cyhalofop-butyl has the potential to induce developmental toxicity, oxidative stress and apoptosis in early life stage of zebrafish (Danio rerio).
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DOI：
10.1016/j.envpol.2015.03.044
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发表时间：
2015-08
影响因子：
8.9
通讯作者：
Lizhen Zhu;Xiyan Mu;Kai Wang;Tingting Chai;Yang Yang-Yang;Lihong Qiu;Chengju Wang
中科院分区：
文献类型：
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作者：
Lizhen Zhu;Xiyan Mu;Kai Wang;Tingting Chai;Yang Yang-Yang;Lihong Qiu;Chengju Wang
氰氟草酯是一种选择性除草剂，广泛应用于水稻田，但可向水体迁移。然而，氰氟草酯的环境风险和水生毒性的细节尚未得到充分的研究。在这项研究中，斑马鱼（Danio rerio）胚胎暴露于一系列氰氟草酯，直到受精后120小时（hpf），以评估胚胎毒性的化学品。我们的研究结果表明，氰氟草酯是高毒性的斑马鱼胚胎，与浓度依赖性的胚胎发育的负面影响。此外，暴露于氰氟草酯导致活性氧（ROS）的生产和细胞凋亡在心脏区域的显着增加。与氧化应激和细胞凋亡相关的基因的mRNA水平也发生了显着变化后，氰氟草酯暴露。此外，capspase-9和caspase-3的活性显著增加。因此，我们推测，氧化应激诱导的细胞凋亡应负责胚胎发育过程中的异常发育后，氰氟草酯曝光。
Cyhalofop-butyl is a selective herbicide widely employed in paddy field, which can transfer into aquatic environments. However, details of the environmental risk and aquatic toxicity of cyhalofop-butyl have not been fully investigated. In this study, zebrafish (Danio rerio) embryos were exposed to a range of cyhalofop-butyl until 120 hour post-fertilization (hpf) to assess embryonic toxicity of the chemical. Our results demonstrated that cyhalofop-butyl was highly toxic to zebrafish embryos, with concentration-dependent negative effects in embryonic development. In addition, exposure to cyhalofop-butyl resulted in significant increases in reactive oxygen species (ROS) production and cell apoptosis in heart area. The mRNA levels of the genes related to oxidative stress and apoptosis were also altered significantly after cyhalofop-butyl exposure. Moreover, the activity of capspase-9 and caspase-3 were significantly increased. Therefore, we speculated that oxidative stress-induced apoptosis should be responsible for abnormal development during embryogenesis after cyhalofop-butyl exposure.