Size Dependent Cellular Uptake of Rod-like Bionanoparticles with Different Aspect Ratios.
Size Dependent Cellular Uptake of Rod-like Bionanoparticles with Different Aspect Ratios.
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DOI：
10.1038/srep24567
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发表时间：
2016-04-15
影响因子：
4.6
通讯作者：
Niu Z
中科院分区：
文献类型：
--
作者：
Liu X;Wu F;Tian Y;Wu M;Zhou Q;Jiang S;Niu Z
了解纳米颗粒的细胞内化机制是研究其生物命运的关键。尤其是由于其各向异性的特性，棒状纳米粒子相对于球形纳米粒子具有更高的内化效率，引起了人们越来越大的兴趣。在这里，为了阐明棒状纳米粒子的长径比对细胞摄取的影响，以典型的棒状生物纳米粒子烟草花叶病毒(TMV)为模型。通过超声波处理和蔗糖密度梯度离心法可以得到不同长径比的纳米棒。通过与上皮细胞和内皮细胞孵育，我们发现不同长径比的棒状生物纳米粒子在不同的细胞系中具有不同的内化途径：TMV4和TMV8在HeLa和HUVEC中的微管转运和网状蛋白介导的摄取；TMV17在HeLa和HUVEC中的小凹介导和微管转运。与大多数纳米粒子不同的是，对于所有三种不同长径比的TMV纳米棒，巨噬细胞吞噬对两种细胞的内化都没有影响。这项工作提供了一个基本的理解长径比对细胞摄取与电荷和材料成分解耦的影响。
Understanding the cellular internalization mechanism of nanoparticles is essential to study their biological fate. Especially, due to the anisotropic properties, rod-like nanoparticles have attracted growing interest for the enhanced internalization efficiency with respect to spherical nanoparticles. Here, to elucidate the effect of aspect ratio of rod-like nanoparticles on cellular uptake, tobacco mosaic virus (TMV), a typical rod-like bionanoparticle, is developed as a model. Nanorods with different aspect ratios can be obtained by ultrasound treatment and sucrose density gradient centrifugation. By incubating with epithelial and endothelial cells, we found that the rod-like bionanoparticles with various aspect ratios had different internalization pathways in different cell lines: microtubules transport in HeLa and clathrin-mediated uptake in HUVEC for TMV4 and TMV8; caveolae-mediated pathway and microtubules transport in HeLa and HUVEC for TMV17. Differently from most nanoparticles, for all the three TMV nano-rods with different aspect ratios, macropinocytosis takes no effect on the internalization in both cell types. This work provides a fundamental understanding of the influence of aspect ratio on cellular uptake decoupled from charge and material composition.