MACRONUCLEAR DNA OF TETRAHYMENA-THERMOPHILA EXISTS AS DEFINED SUB-CHROMOSOMAL-SIZED MOLECULES
MACRONUCLEAR DNA OF TETRAHYMENA-THERMOPHILA EXISTS AS DEFINED SUB-CHROMOSOMAL-SIZED MOLECULES
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DOI：
10.1093/nar/13.16.5817
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发表时间：
1985-01-01
影响因子：
14.9
通讯作者：
YAO, MC
中科院分区：
文献类型：
--
作者：
ALTSCHULER, MI;YAO, MC
用正交场交替凝胶电泳方法，将嗜热四膜虫大核DNA分离成一系列明显的条带。使用电极切换间隔从10到70秒，我们已经解决了DNA带的大小范围从约21 kb的酵母染色体VII和XV的大小。Southern杂交结果表明，T. thermophila有一个精确的组织。每个测试的独特序列只与大核DNA的一条带杂交，并且杂交模式在几个检查的近交系中似乎是相同的。
Using the method of orthogonal-field-alternation gel electrophoresis, we have resolved the macronuclear DNA ofTetrahymena thermophilainto a series of distinct bands. Using electrode switching intervals ranging from 10 to 70 seconds we have resolved DNA bands ranging in size from about 21 kb up to and beyond the size of yeast chromosomes VII and XV. Hybridization of Southern blots from these gels to both unique and repetitive DNA sequences shows that the macronuclear genome ofT. thermophilahas a precise organization. The unique sequences tested each hybridize to only one band of macronuclear DNA and the hybridization patterns seem to be identical in several inbred strains examined.