The effect of ultraviolet radiation exposure on the prevalence of mast cells in human skin
The effect of ultraviolet radiation exposure on the prevalence of mast cells in human skin
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DOI：
10.1046/j.1365-2133.2003.05113.x
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发表时间：
2003-02-01
影响因子：
10.3
通讯作者：
Hart, PH
中科院分区：
文献类型：
--
作者：
Grimbaldeston, MA;Simpson, A;Hart, PH
背景 真皮肥大细胞被认为是先天免疫、超敏反应和紫外线 (UV)B 诱导的细胞介导的接触性过敏原免疫反应抑制中的重要效应细胞。人类与小鼠品系一样，真皮肥大细胞的患病率也存在差异。决定这些差异的因素尚未完全阐明。在小鼠中，真皮肥大细胞上干细胞因子 c-kit 受体的表达与患病率相关。 目的 评估同一供体的未暴露在阳光下和暴露在阳光下的皮肤中的真皮肥大细胞患病率和肥大细胞 c-kit 表达。 方法 在 14 名受试者中，从臀部、手臂内侧、肩部和手背皮肤部位采集皮肤活检 (4 mm)，并对真皮肥大细胞患病率进行量化。评估未暴露在阳光下的臀部和长期暴露在阳光下的手部皮肤中 c-kit 和弹性蛋白含量的肥大细胞表达，这是光老化的特征和紫外线暴露的替代标记。结果手部皮肤中真皮肥大细胞的患病率显着高于其他三个解剖学不同的皮肤部位。在手部皮肤而非臀部皮肤中观察到真皮肥大细胞密度的增加、乳头状真皮中弹性蛋白含量的程度与受试者年龄之间存在显着相关性。 c-kit 的细胞表达与手部皮肤中肥大细胞的流行率相关。然而，在手部皮肤中没有观察到 c-kit 表达、弹性蛋白含量和年龄之间的关系。 结论 人类皮肤中肥大细胞的患病率受到内在因素（调节 c-kit 表达的机制）和外在因素（长期阳光照射）的影响，并且肥大细胞患病率与年龄之间的关系可以通过后者与累积阳光照射的相关性来解释。
Background Dermal mast cells have been implicated as important effector cells in innate immunity, hypersensitivity responses and ultraviolet (UV)B-induced suppression of cell-mediated immune responses to contact allergens. Humans, like mouse strains, display variations in dermal mast cell prevalence. The factors determining these differences are yet to be fully elucidated. In mice, expression of the receptor for stem cell factor, c-kit , on dermal mast cells correlates with prevalence.Objectives To evaluate dermal mast cell prevalence and mast cell c-kit expression in non-sun-exposed and sun-exposed skin in the same donor.Methods In 14 subjects, biopsies of skin (4 mm) were sampled from the skin sites of buttock, inner arm, shoulder and back of hand skin and dermal mast cell prevalence quantified. Non-sun-exposed buttock and chronically sun-exposed hand skin were evaluated for mast cell expression of c-kit and elastin content, a feature of photoageing and surrogate marker of UV exposure.Results The prevalence of dermal mast cells was significantly higher in hand skin than in the three other anatomically different skin sites. Significant correlations were observed in hand but not buttock skin between increasing dermal mast cell densities, extent of elastin content in the papillary dermis and age of the subject. Cellular expression of c-kit correlated with mast cell prevalence in hand skin. However, no relationship was observed in hand skin between c-kit expression, elastin content and age.Conclusions The prevalence of mast cells in human skin is altered by factors that are intrinsic (mechanisms regulating c-kit expression) and extrinsic (chronic sun exposure), and the fact that the associations of mast cell prevalence with age is explained by the latter being a correlate of cumulative sun exposure.