Humulone suppresses replication of respiratory syncytial virus and release of IL-8 and RANTES in normal human nasal epithelial cells
Humulone suppresses replication of respiratory syncytial virus and release of IL-8 and RANTES in normal human nasal epithelial cells
复制标题
DOI：
10.1007/s00795-013-0024-1
复制
发表时间：
2013-12-01
影响因子：
1.8
通讯作者：
Sawada, Norimasa
中科院分区：
文献类型：
--
作者：
Fuchimoto, Jun;Kojima, Takashi;Sawada, Norimasa
呼吸道合胞病毒（RSV）是引起婴幼儿严重呼吸道炎症的主要病原体。然而，尚未开发出针对RSV感染的有效疫苗和抗病毒疗法。啤酒花衍生的苦味酸对炎症具有有效的药理作用。因此，我们研究了蛇麻酮，这是啤酒花苦酸的主要成分，对RSV的复制和释放的促炎细胞因子IL-8和趋化因子RANTES在RSV感染的人鼻上皮细胞（HNECs）的影响。我们发现，在RSV感染的HNECs中，蛇麻酮以剂量依赖的方式阻止RSV/G蛋白的表达、病毒丝的形成以及IL-8和RANTES的释放。这些结果表明，蛇麻酮对HNECs中的RSV复制、病毒组装和炎症反应具有保护作用，并且蛇麻酮是用于预防和治疗RSV感染的有用的生物制品。
Respiratory syncytial virus (RSV) is the major infectious agent causing serious respiratory tract inflammation in infants and young children. However, an effective vaccine and anti-viral therapy for RSV infection have not yet been developed. Hop-derived bitter acids have potent pharmacological effects on inflammation. Therefore, we investigated the effects of humulone, which is the main constituent of hop bitter acids, on the replication of RSV and release of the proinflammatory cytokine IL-8 and chemokine RANTES in RSV-infected human nasal epithelial cells (HNECs). We found that humulone prevented the expression of RSV/G-protein, formation of virus filaments and release of IL-8 and RANTES in a dose-dependent manner in RSV-infected HNECs. These findings suggest that humulone has protective effects against the replication of RSV, the virus assembly and the inflammatory responses in HNECs and that it is a useful biological product for the prevention and therapy for RSV infection.