Moderate-density molecular maps of Eucalyptus urophylla S. T.!Blake and E-tereticornis Smith genomes based on RAPD markers
Moderate-density molecular maps of Eucalyptus urophylla S. T.!Blake and E-tereticornis Smith genomes based on RAPD markers
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DOI：
10.1023/a:1022966018079
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发表时间：
2003-05-01
期刊：
影响因子：
1.5
通讯作者：
Bai, JY
中科院分区：
文献类型：
--
作者：
Gan, SM;Shi, JS;Bai, JY
构建了尾叶桉（Eucalyptus urophylla S.）基因组的中密度分子图谱。T. Blake和E. tereticornis Smith的RAPD标记和种间杂交。利用183个引物在E. urophylla和E. tereticornis，以及49个父母共享的标记。在正常分离的标记中，有208个（84.9%）为母本E. urophylla，175株（66.3%）为父本E. tereticornis和双亲共有的48个用于每个亲本物种的框架图构建。对于母本E.该连锁图由23个连锁群、160个框架标记和60个辅助标记组成，图距为1504.6cM，平均图距为11.0 ± 8.07cM。对于父系E.该连锁图包含23个连锁群，126个框架标记和92个辅助标记，总图距为1035.7cM，平均图距为10.1 ± 7.23cM。基因组长度分别为1585.7和1507.5cM。urophylla和E. tereticornis，分别表示94.9%和68.7%的相应基因组的地图覆盖率。这些图谱的构建对于桉树育种中的数量性状位点（QTL）检测、标记辅助选择（MAS）、比较作图和基于全基因组的指纹图谱分析具有重要意义。
Moderate-density molecular maps were constructed for the genomes of Eucalyptus urophylla S. T. Blake and E. tereticornis Smith using RAPD markers and an interspecific cross between the two species. One hundred and eighty-three primers were employed to generate 245 and 264 parent-specific markers in E. urophylla and E. tereticornis, respectively, as well as 49 parent-shared markers. The normally segregating markers, including 208 (84.9%) specific to maternal E. urophylla, 175 (66.3%) to paternal E. tereticornis, and 48 shared by both parents, were used for framework map construction for each parental species. For maternal E. urophylla, the linkage map consisted of 23 linkage groups, 160 framework markers, and 60 accessory markers, defining a total map distance of 1504.6 cM and an average interval of 11.0 +/- 8.07 cM. For paternal E. tereticornis, the linkage map contained 23 linkage groups, 126 framework markers, and 92 accessory markers, defining a total map distance of 1035.7 cM and an average interval of 10.1 +/- 7.23 cM. Genome length was estimated at 1585.7 and 1507.5 cM for E. urophylla and E. tereticornis, respectively, indicating map coverage of 94.9 and 68.7% of the corresponding genomes. Construction of such maps will be valuable for quantitative trait loci (QTLs) detection, marker-assisted selection (MAS), comparative mapping, and whole genome based fingerprint characterization in Eucalyptus breeding programs.