ARNT-deficient mice and placental differentiation
ARNT-deficient mice and placental differentiation
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DOI：
10.1006/dbio.1997.8758
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发表时间：
1997-11-15
影响因子：
2.7
通讯作者：
Hankinson, O
中科院分区：
文献类型：
--
作者：
Kozak, KR;Abbott, B;Hankinson, O
我们使用胚胎干细胞中的同源重组来产生芳烃核转位器（ARNT）无效突变的杂合子小鼠。这些小鼠进行了杂交，但在64只新生儿中没有产生活的纯合Amt -/-敲除小鼠。纯合子在妊娠9.5至10.5天之间死于子宫内。流产包括神经管闭合缺陷、前脑发育不全、胚胎旋转延迟、胎盘老化和内脏弓异常。然而，致死的主要原因似乎是胎盘的胚胎成分未能血管化和形成胎盘海绵滋养层。这可能与ARNT在缺氧诱导血管生成中的已知作用有关。我们没有发现卵黄囊循环的缺陷。(C)北京：科学出版社.
We used homologous recombination in embryonic stem cells to generate mice heterozygous for an aryl hydrocarbon nuclear translocator (ARNT) null mutation. These mice were intercrossed, but no live homozygous Amt -/- knockout mice were produced among 64 newborns. Homozygotes die in utero between 9.5 and 10.5 days of gestation. Abnormalities included neural tube closure defects, forebrain hypoplasia, delayed rotation of the embryo, placental hemorrhaging, and visceral arch abnormalities. However, the primary cause of lethality appears to be failure of the embryonic component of the placenta to vascularize and form the labyrinthine spongiotrophoblast. This may be related to ARNT's known role in hypoxic induction of angiogenesis. We found no defects in yolk sac circulation. (C) 1997 Academic Press.