In vivo measurement of retinal physiology with high-speed ultrahigh-resolution optical coherence tomography
In vivo measurement of retinal physiology with high-speed ultrahigh-resolution optical coherence tomography
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DOI：
10.1364/ol.31.002308
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发表时间：
2006-08-01
期刊：
影响因子：
3.6
通讯作者：
Duker, J. S.
中科院分区：
文献类型：
--
作者：
Srinivasan, V. J.;Wojtkowski, M.;Duker, J. S.
利用高速、超高分辨率光学相干断层扫描（OCT）展示了完整视网膜的无创体内功能光学成像。成像以2.8 μ m分辨率进行，每秒24,000次轴向扫描。在适应黑暗的大鼠视网膜上施加白光刺激，并监测不同视网膜层的平均反射率随时间的变化。光感受器外节的平均反射率增加10%-15%。OCT信号变化的空间分布与光感受器外段后向散射的增加一致。据我们所知，这是第一次在完整视网膜上进行OCT功能成像的活体演示。(c) 2006年美国光学学会。
Noninvasive in vivo functional optical imaging of the intact retina is demonstrated by using high-speed, ultrahigh-resolution optical coherence tomography (OCT). Imaging was performed with 2.8 mu m resolution at a rate of 24,000 axial scans per second. A white-light stimulus was applied to the dark-adapted rat retina, and the average reflectivities from different intraretinal layers were monitored as a function of time. A 10%-15% increase in the average amplitude reflectance of the photoreceptor outer segments was observed in response to the stimulus. The spatial distribution of the change in the OCT signal is consistent with an increase in backscatter from the photoreceptor outer segments. To our knowledge, this is the first in vivo demonstration of OCT functional imaging in the intact retina. (c) 2006 Optical Society of America.