Direct visualization of site-specific and strand-specific DNA methylation patterns in automated DNA sequencing data
Direct visualization of site-specific and strand-specific DNA methylation patterns in automated DNA sequencing data
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DOI：
10.1093/nar/26.10.2505
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发表时间：
1998-05-15
影响因子：
14.9
通讯作者：
Buckler-White, A
中科院分区：
文献类型：
--
作者：
Rao, BS;Buckler-White, A
我们在这里报告了一种简单的方法，可以在自动 DNA 测序色谱图中直接可视化不同类型的 DNA 甲基化，包括 Hpall 和 dcm 位点中的胞嘧啶甲基化以及 dam 位点中的腺嘌呤甲基化。这是通过观察到荧光标记的双脱氧核苷酸的掺入程度受到模板 DNA 中甲基化碱基的影响而实现的。这种简单的方法涉及常规自动 DNA 测序，无需事先对 DNA 进行任何专门用于检测 DNA 甲基化的处理。
We report here a simple method of directly visualizing in automated DNA sequencing chromatograms DNA methylations of different types including cytosine methylations in Hpall and dcm sites as well as adenine methylations in dam sites. This is made possible by the observation that the extent of incorporation of fluorescently labeled dideoxynucleotides is influenced by the methylated bases in template DNA. This simple approach involves routine automated DNA sequencing without any prior treatment of DNA specific for detecting DNA methylation.