Optimal chlorophyll fluorescence parameter selection for rapid and sensitive detection of lead toxicity to marine microalgae Nitzschia closterium based on chlorophyll fluorescence technology
Optimal chlorophyll fluorescence parameter selection for rapid and sensitive detection of lead toxicity to marine microalgae Nitzschia closterium based on chlorophyll fluorescence technology
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基于叶绿素荧光技术的最佳叶绿素荧光参数选择快速灵敏检测海洋微藻新月菱形藻铅毒性
DOI：
10.1016/j.jphotobiol.2019.111551
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发表时间：
2019-08-01
影响因子：
5.4
通讯作者：
Liu, Wenqing
中科院分区：
文献类型：
--
作者：
Gan, Tingting;Zhao, Nanjing;Liu, Wenqing
重金属污染是海洋环境中最严重的污染问题之一，严重威胁着海洋生物的安全和人类的健康，并对海洋生态环境造成潜在的风险。为了建立一种快速、灵敏的海洋重金属毒性检测方法，以海洋硅藻新月菱形藻（Nitzschia closterium）为试验生物，研究了不同浓度铅（Pb）对新月菱形藻5种叶绿素荧光参数的影响。利用叶绿素荧光技术，研究了不同光照时间下新月藻的光系统Ⅱ（PSII）最大光化学量子效率（Fv/Fm）、PSII光化学能量转换有效量子效率（Phi（PSII））、PSII光化学有效吸收截面（sigma（PSII））、PSII相对电子传递速率（rP）和单位体积电子通量（JVPII）。以藻类为试验生物，通过与污染物毒性分析常用的光合活性荧光参数Fv/Fm的比较，筛选出了快速、灵敏测定Pb对N.选择新月形。结果表明，在8 h内，叶绿素荧光参数Fv/Fm、Phi（PSII）sigma（PSII）、rP和JVPII均与Pb具有良好的剂量-反应关系，可作为快速测定Pb对N毒害的指标。新月体在5个叶绿素荧光参数中，JVPII是检测Pb对N毒害最敏感的荧光参数。暴露6 h内的新月体。JVP Ⅱ在2、4和6 h的半数有效浓度（EC_（50））分别为0.329、0.068和0.040 mmol L ~（-1）。而当暴露时间为8 h时，Phi（PSII）是检测Pb毒性最灵敏的荧光参数，Pb在8 h的EC 50值为0.038 mmol L-1。该研究为建立快速、灵敏的海洋重金属生物毒性检测方法提供了重要依据，也为海洋环境生态风险评价提供了参考。
Heavy metal pollution as one of the most serious pollution problems of marine environment, seriously threatens the safety of marine organism and human health, and will lead to potential risks for the marine ecological environment. In order to develop a rapid and sensitive toxicity detection method for marine heavy metals, in this study, marine diatom Nitzschia closterium was used as the test organism, and the effects of different concentrations of lead (Pb) on the five chlorophyll fluorescence parameters of N. closterium including the maximum photochemical quantum yield of PSII (Fv/Fm), the effective quantum yield of PSII photochemical energy conversion (Phi(PSII)), the effective absorption cross section of PSII photochemistry (sigma(PSII)), the relative electron transfer rate of PSII (rP), and the PSII electron flux per unit volume (JVPII) at different exposure times were investigated based on chlorophyll fluorescence technology. By comparing with the photosynthetic activity fluorescence parameter Fv/Fm which is commonly used for toxicity analysis of pollutants using algae as test organisms, the optimal chlorophyll fluorescence parameter that could rapidly and sensitively determine Pb toxicity to N. closterium was selected. The results indicate that all the five chlorophyll fluorescence parameters of Fv/Fm, Phi(PSII) sigma(PSII)', rP and JVPII showed good dose-response relationships with Pb within 8 h exposure time, and they all could be used as endpoints to rapidly determine Pb toxicity to N. closterium. Among the five chlorophyll fluorescence parameters, JVPII was the most sensitive fluorescence parameter for detecting the toxicity of Pb to N. closterium within 6 h exposure. And for JVPII, the median effective concentration (EC50) values of Pb at 2, 4 and 6 h were 0.329, 0.068 and 0.040 mmol L-1, respectively. However, when the exposure time was 8 h, Phi(PSII) was the most sensitive fluorescence parameter for the toxicity detection of Pb, and the EC50 value of Pb at 8 h was 0.038 mmol L-1. This study will provide an important basis for the development of a rapid and sensitive detection method for the biological toxicity of marine heavy metals, and those results will be helpful for ecological risk assessment in marine environment.