Activation of an EDS1-mediated R-gene pathway in the snc1 mutant leads to constitutive, NPR1-independent pathogen resistance
Activation of an EDS1-mediated R-gene pathway in the snc1 mutant leads to constitutive, NPR1-independent pathogen resistance
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DOI：
10.1094/mpmi.2001.14.10.1131
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发表时间：
2001-10-01
影响因子：
3.5
通讯作者：
Dong, XN
中科院分区：
文献类型：
--
作者：
Li, X;Clarke, JD;Dong, XN
拟南芥NPR1蛋白是系统获得性抗性(SAR)的重要调节成分。NPR1基因的突变完全阻断了水杨酸(SA)等信号对SAR的诱导。在npr1-1抑制子的筛选中分离到一个拟南芥突变体SIZE](npr1-1的抑制子，组成部分1)。在npr1-1背景下，SNc1突变导致了对黄斑假单胞菌ES4326和寄生轮孢菌Noco2的结构性抗性。在突变体中检测到高水平的SA，并表明这是表现Site]表型所必需的。Snc1突变被定位在RPP5抗性(R)基因簇上，而阻断RPP5介导的抗性的eds1突变抑制了Snc1。这些数据表明，在SNc1中，RPP5相关的抗性通路被结构性地激活。该途径不使用NPR1。但需要信号分子SA和EDS1的功能。此外，在SNc1中，结构性抗性是在没有细胞死亡的情况下产生的，这通常与R基因介导的抗性有关。
The Arabidopsis NPR1 protein is an essential regulatory component of systemic acquired resistance (SAR). Mutations in the NPR1 gene completely block the induction of SAR by signals such as salicylic acid (SA). An Arabidopsis mutant, size] (suppressor of npr1-1, constitutive 1), was isolated in a screen for suppressors of npr1-1. In the npr1-1 background, the snc1 mutation resulted in constitutive resistance to Pseudomonas syringae maculicola ES4326 and Peronospora parasitica Noco2. High levels of SA were detected in the mutant and shown to be required for manifestation of the site] phenotype. The snc1 mutation was mapped to the RPP5 resistance (R) gene cluster and the eds1 mutation that blocks RPP5-mediated resistance suppressed snc1. These data suggest that a RPP5-related resistance pathway is activated constitutively in snc1. This pathway does not employ NPR1. but requires the signal molecule SA and the function of EDS1. Moreover, in snc1, constitutive resistance is conferred in the absence of cell death, which is often associated with R-gene mediated resistance.