Kinetics of Cytokine mRNA Expression in the Central Nervous System Following Lethal and Nonlethal Coronavirus-Induced Acute Encephalomyelitis
Kinetics of Cytokine mRNA Expression in the Central Nervous System Following Lethal and Nonlethal Coronavirus-Induced Acute Encephalomyelitis
复制标题
致死性和非致死性冠状病毒引起的急性脑脊髓炎后中枢神经系统细胞因子 mRNA 表达的动力学
作者：
B. Parra;D. Hinton;Mark T. Lin;D. Cua;S. Stohlman
摘要
通过测定小鼠肝炎病毒嗜神经性JHM株感染后细胞因子mRNA表达的动力学，检查细胞因子在减少中枢神经系统中感染性病毒和持续性病毒感染中的潜在作用。用产生非致死性脑脊髓炎的抗体逃逸变体感染小鼠，并与产生暴发性致死性脑脊髓炎的克隆病毒群体进行比较。两种病毒感染均诱导与Th 1和Th 2型细胞因子（包括IFN-γ、IL-4和IL-10）相关的mRNA积累。mRNA的峰值积累与病毒的清除相一致，致死性和非致死性感染小鼠之间无明显差异。与非致死性脑脊髓炎小鼠相比，致死性感染小鼠中TNF-α mRNA的诱导更快。感染后，编码IL-12、iNOS、IL-1α、IL-1β和IL-6的mRNA迅速瞬时增加。非致死性感染与IL-12、IL-1β增加和IL-6早期表达相关，而致死性感染与iNOS和IL-1α mRNA增加相关。这些数据表明中枢神经系统细胞对不同JHMV变体感染的反应迅速但有差异。然而，无论是诱导的积累还是动力学都不能提供区分致死性感染和导致持续性感染的非致死性感染的证据。JHMV感染小鼠中枢神经系统中Th 1和Th 2细胞因子的积累与急性病毒诱导的脑脊髓炎消退期间细胞因子和细胞免疫效应物的参与一致。
Abstract
The potential role(s) of cytokines in the reduction of infectious virus and persistent viral infection in the central nervous system was examined by determining the kinetics of cytokine mRNA expression following infection with the neurotropic JHM strain of mouse hepatitis virus. Mice were infected with an antibody escape variant which produces a nonlethal encephalomyelitis and compared to a clonal virus population which produces a fulminant fatal encephalomyelitis. Infection with both viruses induced the accumulation of mRNAs associated with Th1- and Th2-type cytokines, including IFN-γ, IL-4, and IL-10. Peak mRNA accumulations were coincident with the clearance of virus and there was no obvious differences between lethally and nonlethally infected mice. TNF-α mRNA was induced more rapidly in lethally infected mice compared to mice undergoing a nonfatal encephalomyelitis. Rapid transient increases in the mRNAs encoding IL-12, iNOS, IL-1α, IL-1β, and IL-6 occurred following infection. Nonlethal infections were associated with increased IL-12, IL-1β, and earlier expression of IL-6, while lethal infections were associated with increased iNOS and IL-1α mRNA. These data suggest a rapid but differential response within the central nervous system cells to infection by different JHMV variants. However, neither the accumulation nor kinetics of induction provide evidence to distinguish lethal infections from nonlethal infections leading to a persistent infection. Accumulation of both Th1 and Th2 cytokines in the central nervous system of JHMV-infected mice is consistent with the participation of both cytokines and cell immune effectors during resolution of acute viral-induced encephalomyelitis.
小鼠内毒素血症期间 IL-12 的产生不依赖于 IFN-γ。
DOI：
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发表时间：
1996
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Heinzel,FP;Rerko,RM;Ahmed,F;Hujer,AM
通讯作者：
Hujer,AM
致命性和非致命性甲病毒脑炎期间的脑内细胞因子 mRNA 表达表明主要是 2 型 T 细胞反应。
DOI：
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发表时间：
1994
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
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作者：
Wesselingh,SL;Levine,B;Fox,RJ;Choi,S;Griffin,DE
通讯作者：
Griffin,DE