Evaluating the core microbiota in complex communities: A systematic investigation
Evaluating the core microbiota in complex communities: A systematic investigation
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DOI：
10.1111/1462-2920.13647
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发表时间：
2017-04-01
影响因子：
5.1
通讯作者：
Taylor, Michael W.
中科院分区：
文献类型：
--
作者：
Astudillo-Garcia, Carmen;Bell, James J.;Taylor, Michael W.
复杂微生物群落的研究提出了独特的概念和分析挑战，微生物物种可能数以千计。由于瞬时或外来微生物往往会增加这种复杂性，因此只关注社区中稳定和永久成员的核心微生物群方法越来越受欢迎。考虑到定义核心微生物群的各种方法，谨慎的做法是检查核心的定义是否会影响所获得的结果。在这里，我们使用复杂的海绵微生物，并进行了系统的评估，在何种程度上不同的因素用于定义核心的影响的结论。使用一些但不是所有的核心定义检测到α和β多样性的显着差异。然而，与宿主特异性和环境质量相关的研究结果对核心微生物群定义的重大变化基本不敏感。此外，没有一个应用的定义改变了我们对生态网络的看法，生态网络总结了海绵中细菌之间的相互作用。这些结果表明，虽然在解释时仍应小心，但核心微生物群方法令人惊讶地稳健，至少对于比较密切相关样品的微生物群而言。
The study of complex microbial communities poses unique conceptual and analytical challenges, with microbial species potentially numbering in the thousands. With transient or allochthonous microorganisms often adding to this complexity, a core' microbiota approach, focusing only on the stable and permanent members of the community, is becoming increasingly popular. Given the various ways of defining a core microbiota, it is prudent to examine whether the definition of the core impacts upon the results obtained. Here we used complex marine sponge microbiotas and undertook a systematic evaluation of the degree to which different factors used to define the core influenced the conclusions. Significant differences in alpha- and beta-diversity were detected using some but not all core definitions. However, findings related to host specificity and environmental quality were largely insensitive to major changes in the core microbiota definition. Furthermore, none of the applied definitions altered our perception of the ecological networks summarising interactions among bacteria within the sponges. These results suggest that, while care should still be taken in interpretation, the core microbiota approach is surprisingly robust, at least for comparing microbiotas of closely related samples.