Short report: An agar plate method for culturing hookworm larvae: Analysis of growth kinetics and infectivity compared with standard coproculture techniques
Short report: An agar plate method for culturing hookworm larvae: Analysis of growth kinetics and infectivity compared with standard coproculture techniques
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DOI：
10.4269/ajtmh.2007.77.1087
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发表时间：
2007-12-01
影响因子：
3.3
通讯作者：
Cappello, Michael
中科院分区：
文献类型：
--
作者：
Reiss, Daniel;Harrison, Lisa M.;Cappello, Michael
An agar plate (AP) method has been developed for culturing infectious larvae of the hookworm Ancylostoma ceylanicum. The third-stage larvae reared using the AP method displayed similar morphology to those cultured using Baermann or Harada-Mori coproculture techniques. The yield of viable larvae from the AP method (50%) was comparable to that of the Baermann (47%), and both were superior to Harada-Mori (2.1%). Third-stage larvae cultured by the AP method established patent infection in naturally permissive laboratory hosts, although the yield of adult worms was reduced compared with animals infected with L3 obtained by Baermann culture. The AP method is useful for defining growth requirements for hookworm development, as well as characterizing the effects of bacterially expressed compounds on hookworm larvae in vivo.
