Bacteriophage lambda is a highly stable DNA vaccine delivery vehicle
Bacteriophage lambda is a highly stable DNA vaccine delivery vehicle
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DOI：
10.1016/j.vaccine.2003.11.065
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发表时间：
2004-06-23
期刊：
影响因子：
5.5
通讯作者：
March, JB
中科院分区：
文献类型：
--
作者：
Jepson, CD;March, JB
已检查了用作DNA疫苗递送系统的完整噬菌体X颗粒的稳定性。发现噬菌体在正常储存条件下高度稳定。在液体悬浮液中，在4 ℃和-70 ℃下6个月内没有观察到滴度降低，并且噬菌体稳定性不受冻融影响。在20 ℃、37 ℃和42 ℃下测定的噬菌体在悬浮液中的半衰期分别为36天、3.4天和2.3天。冷冻干燥的噬菌体悬浮液（有或没有稳定剂脱脂奶粉或海藻糖）导致5-20%的残留活力。干燥后（有或没有稳定剂），测得的半衰期范围为20至100天（20 ℃）、2.6至38天（37 ℃）、2.1至26天（42 ℃）、7至33小时（70 ℃）和1.3至6分钟（100 ℃）。在所有情况下，海藻糖的加入显著增加了干燥噬菌体的稳定性。当在-70 ℃下储存时，干燥的噬菌体在没有稳定剂的情况下似乎是稳定的。当噬菌体X稀释到水中时，在2周的时间内观察到滴度的边际损失。在24 It的时间内，液体噬菌体悬浮液在pH范围F-H 3-11内保持稳定，因此通过饮用水口服噬菌体DNA疫苗是可能的。(C)2004 Elsevier Ltd.保留所有权利。
The stability of whole bacteriophage X particles, used as a DNA vaccine delivery system has been examined. Phage were found to be highly stable undernormal storage conditions. In liquid suspension, no decrease in titre was observed over a 6-month period at 4 and -70degreesC, and phage stability was unaffected by freeze/thawing. The measured half life of phage in suspension was 36 days at 20degreesC, 3.4 days at 37degreesC and 2.3 days at 42degreesC. Freeze drying of a phage suspension (with or without the stabilizers dry skim milk or trehalose) resulted in 5-20% residual viability. Following desiccation (with or without stabilizers), measured half lives ranged from 20 to 100 days at 20degreesC, 2.6 to 38 days at 37degreesC, 2.1 to 26 days at 42degreesC, 7 to 33 h at 70degreesC, and 1.3 to 6 m at 100degreesC. In all cases the addition of trehalose significantly increased the stability of the desiccated phage. When stored at -70degreesC, desiccated phage appeared to be stable in the absence of stabilizers. When phage X was diluted into water, a marginal loss in titre was observed over a 2-week period. Over a 24 It period, liquid phage suspensions were stable within the pH range F-H 3-11, therefore oral administration of bacteriophage DNA vaccines via drinking water may be possible. (C) 2004 Elsevier Ltd. All rights reserved.