Virulent and nonvirulent Flavobacterium columnare colony morphologies: characterization of chondroitin AC lyase activity and adhesion to polystyrene
Virulent and nonvirulent Flavobacterium columnare colony morphologies: characterization of chondroitin AC lyase activity and adhesion to polystyrene
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DOI：
10.1111/j.1365-2672.2011.05149.x
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发表时间：
2011-12-01
影响因子：
4
通讯作者：
Sundberg, L. -R.
中科院分区：
文献类型：
--
作者：
Kunttu, H. M. T.;Jokinen, E. I.;Sundberg, L. -R.
目的：对鱼类致病性柱状黄杆菌的菌落形态变异进行了研究，以阐明菌落形态变化在细菌毒力中的作用。方法和结果：研究了不同温度下和细菌表面修饰后，不同菌株的软骨素AC裂解酶活性、对聚苯乙烯的粘附性和脂多糖（LPS）谱。的软骨素酶活性显着较高的毒性，假根的变种比在同一菌株的粗糙的变种。温度显著增加假根变体的粘附性，高达20摄氏度。细菌表面的修饰表明，粘附分子含有碳水化合物和蛋白质。LPS没有不同的变种之间的同一strain.Conclusions：结果表明，在Fl. columare两个假根菌落形态和高软骨素酶活性的毒力和温度可能会促进有毒的变种附着到表面在养鱼场。关于柱状芽孢杆菌的毒力机制以及该细菌在养鱼场存活的原因产生了新的信息。
Aims: Colony morphology variants of fish pathogenic Flavobacterium columnare were studied to clarify the role of colony morphology change in the virulence of the bacterium. Typical rhizoid colony (Rz) variants are virulent and moderately adherent, nonrhizoid rough (R) colony variants are nonvirulent and highly adherent, and soft colony (S) variants are nonvirulent and poorly adherent.Methods and Results: Chondroitin AC lyase activity, adhesion to polystyrene at different temperatures and after modification of bacterial surface, and lipopolysaccharide (LPS) profiles of the variants were studied. The chondroitinase activity was significantly higher in the virulent, rhizoid variants than in the rough variants of the same strain. Temperature significantly increased the adhesion of rhizoid variants up to 20 degrees C. Modification of bacterial surface suggested that adhesion molecules contain both carbohydrates and proteins. LPS did not differ between the variants of the same strain.Conclusions: The results suggest that in Fl. columnare both rhizoid colony morphology and high chondroitinase activity are needed for virulence and that temperature may promote the adhesion of the virulent variants to surfaces at fish farms.Significance and Impact of the Study: New information is produced on the virulence mechanisms of Fl. columnare and the reasons behind the survival of the bacterium at fish farms.