PURIFICATION OF CHLAMYDIA-TRACHOMATIS BY A SIMPLE AND RAPID FILTRATION METHOD
PURIFICATION OF CHLAMYDIA-TRACHOMATIS BY A SIMPLE AND RAPID FILTRATION METHOD
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DOI：
10.1099/00221287-137-7-1565
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发表时间：
1991-07-01
期刊：
影响因子：
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通讯作者：
RICHMOND, SJ
中科院分区：
文献类型：
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作者：
CAMPBELL, S;YATES, PS;RICHMOND, SJ
本文介绍一种从细胞培养物中过滤纯化沙眼衣原体的简单方法。 使衣原体感染细胞的粗匀浆通过玻璃预滤器和0.6 μ m孔径的聚碳酸酯滤器。 然后使滤液通过孔径为0.2 μ m的过滤器，在该过滤器上捕获衣原体。 然后反洗该过滤器以收集微生物。 这些程序去除了细胞碎片和可溶性蛋白质，并产生具有窄尺寸分布的颗粒。 当在该过程的每个阶段洗涤过滤器时，通过过滤纯化的活衣原体的平均产率为64%。
A simple method for filter purification of Chlamydia trachomatis from cell culture is described. Crude homogenates of chlamydiae-infected cells were passed through a glass prefilter and a 0.6-mu-m pore diameter polycarbonate filter. The filtrate was then passed through a 0.2-mu-m pore diameter filter on which the chlamydiae were trapped. This filter was then back-washed to collect the organisms. These procedures removed cell debris and soluble protein, and yielded particles with a narrow size distribution. The mean yield of viable chlamydiae purified by filtration was 64% when the filters were washed at each stage of the process.